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Literature summary extracted from
- Acyltransferase activity of the wide spectrum amidase of Brevibacterium sp. R312 (1986), J. Gen. Microbiol., 132, 2205-2208.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.4 | 16 | - |
propionic acid ethyl ester | - |
Brevibacterium sp. |  |
| 3.5.1.4 | 18 | - |
ethyl acetate | - |
Brevibacterium sp. | |
| 3.5.1.4 | 31 | - |
acetamide | - |
Brevibacterium sp. | |
| 3.5.1.4 | 83 | - |
acetate | - |
Brevibacterium sp. | |
| 3.5.1.4 | 88 | - |
Propionamide | - |
Brevibacterium sp. | |
| 3.5.1.4 | 93 | - |
Acrylamide | - |
Brevibacterium sp. | |
| 3.5.1.4 | 218 | - |
ethyl prop-2-enoate | - |
Brevibacterium sp. | |
| 3.5.1.4 | 628 | - |
propionate | - |
Brevibacterium sp. | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | ethyl acetate + H2O | Brevibacterium sp. | - |
ethanol + acetate | - |
? | |
| 3.5.1.4 | ethyl acrylate + H2O | Brevibacterium sp. | - |
acrylate + ethanol | - |
? | |
| 3.5.1.4 | ethyl propionate + H2O | Brevibacterium sp. | - |
ethanol + propionate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.4 | Brevibacterium sp. | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | additional information | - |
in the presence of hydroxylamine, the amidase would convert acetamide to acetohydroxamic acid rather than to ammonium acetate | Brevibacterium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | acetamide + H2O | - |
Brevibacterium sp. | acetate + NH3 | - |
? | |
| 3.5.1.4 | acrylamide + H2O | - |
Brevibacterium sp. | acrylate + NH3 | - |
? | |
| 3.5.1.4 | ethyl acetate + H2O | - |
Brevibacterium sp. | ethanol + acetate | - |
? | |
| 3.5.1.4 | ethyl acrylate + H2O | - |
Brevibacterium sp. | acrylate + ethanol | - |
? | |
| 3.5.1.4 | ethyl propionate + H2O | - |
Brevibacterium sp. | ethanol + propionate | - |
? | |
| 3.5.1.4 | propionamide + H2O | - |
Brevibacterium sp. | propionate + NH3 | - |
? | |
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