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Literature summary extracted from

  • Gregoriou, M.; Brown, P.R.
    Inhibition of the aliphatic amidase from Pseudomonas aeruginosa by urea and related compounds (1979), Eur. J. Biochem., 96, 101-108.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 acetaldehyde competitive inhibitor, not time dependent; competitve inhibitor, Ki: 7.0 micromol Pseudomonas aeruginosa
3.5.1.4 acetaldehyde ammonia
-
 Pseudomonas aeruginosa
3.5.1.4 Cyanate Ki of strain AI3 is 1.1 mM, Ki of strain AIU1N is 1.8 mM and Ki of strain OUCH4 is 50 mM; reversible inhibition, pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 Hydroxyurea Ki of strain AI3 is 1.4 mM, Ki of strain AIU1N is 10.8 mM and Ki of strain OUCH4 is 337 mM, regaines full activity within 24 h at 37°C in 0.05 M Tris buffer at pH 7.2; reversible inhibition, pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 additional information no inhibition with 10 mM guanidinium carbonate, 100 mM thiourea, ammonium carbamate at strain AI 3 Pseudomonas aeruginosa
3.5.1.4 Urea competitive inhibitor at pH 7.2, 90 mM acetamide protectes AI3 amidase against 2.9 mM urea over 2 h, Ki of strain AI3 is 6.6 mM, Ki of strain AIU1N is 41.3 mM and Ki of strain OUCH4 is not known, regaines full activity within 24 h at 37°C in 0.05 M Tris buffer at pH 7.2; reversible inhibition, pH 7.2 and 37°C Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 68.6
-
 acetamide strain AI3 Pseudomonas aeruginosa
3.5.1.4 90.4
-
 acetamide strain AIU1N Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 41600
-
 6 * 41600, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 240000
-
 SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 240000
-
 calculated from measurement of the subunit molecular weight, strain AI3 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 acetanilide + H2O Pseudomonas aeruginosa
-
 acetate + aniline
-
 ?
3.5.1.4 acetanilide + H2O Pseudomonas aeruginosa AI3
-
 acetate + aniline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Pseudomonas aeruginosa
-
 and the mutant strains AIU1N and OUCH4
-
3.5.1.4 Pseudomonas aeruginosa
-
 and strains AIU1N and OUCH4
-
3.5.1.4 Pseudomonas aeruginosa AI3
-
 and the mutant strains AIU1N and OUCH4
-
3.5.1.4 Pseudomonas aeruginosa AI3
-
 and strains AIU1N and OUCH4
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 0.8
-
 at 3 mM formamide, formamidase activity of AI3 amidase Pseudomonas aeruginosa
3.5.1.4 1.5
-
 substrate 4-nitro-acetanilide, strain OUCH4 Pseudomonas aeruginosa
3.5.1.4 1.5
-
 4-nitroacetanilide, strain OUCH 4, pH 8.5 Pseudomonas aeruginosa
3.5.1.4 7.4
-
 substrate 4-nitro-acetanilide, strain AIU1N Pseudomonas aeruginosa
3.5.1.4 7.4
-
 4-nitroacetanilide, strain AIU 1N, pH 8.5 Pseudomonas aeruginosa
3.5.1.4 10.5
-
 substrate 4-nitro-acetanilide, strain AI3 25-fold greater activity than wild-type Pseudomonas aeruginosa
3.5.1.4 10.5
-
 4-nitroacetanilide, strain AI3, pH 8.5 Pseudomonas aeruginosa
3.5.1.4 2900
-
 substrate acetamide, hydrolysis, strain AIU1N Pseudomonas aeruginosa
3.5.1.4 4100
-
 substrate acetamide, hydrolysis, strain AI3 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
3.5.1.4 -30°C, 0.05 M Tris buffer, pH 7.2, 50% v/v glycerol, 1 mM 2-mercaptoethanol Pseudomonas aeruginosa
3.5.1.4 purified amidase stored in 0.05 M Tris buffer, pH 7.2, containing 1 mM mercaptoethanol and 50%, v/v, glycerol, at -30°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 4-nitroacetanilide + H2O
-
 Pseudomonas aeruginosa acetic acid + 4-nitroaniline
-
 ?
3.5.1.4 4-nitroacetanilide + H2O
-
 Pseudomonas aeruginosa AI3 acetic acid + 4-nitroaniline
-
 ?
3.5.1.4 acetamide + H2O
-
 Pseudomonas aeruginosa acetic acid + NH3
-
 ?
3.5.1.4 acetamide + H2O
-
 Pseudomonas aeruginosa AI3 acetic acid + NH3
-
 ?
3.5.1.4 acetanilide + H2O
-
 Pseudomonas aeruginosa acetate + aniline
-
 ?
3.5.1.4 acetanilide + H2O
-
 Pseudomonas aeruginosa AI3 acetate + aniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 hexamer 6 * 41600, SDS-PAGE Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 0.143
-
 acetamide hydrolysis, strain AI3 Pseudomonas aeruginosa
3.5.1.4 1.51
-
 acetamide hydrolysis, strain AIU1N Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.4 0.005
-
 acetaldehyde strain AI3 Pseudomonas aeruginosa
3.5.1.4 0.0051
-
 acetaldehyde competitive inhibitor Pseudomonas aeruginosa
3.5.1.4 0.007
-
 acetaldehyde ammonia strain AI3 Pseudomonas aeruginosa
3.5.1.4 1.1
-
 Cyanate strain AI3, pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 1.4
-
 Hydroxyurea strain AI3, pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 1.8
-
 Cyanate strain AIU1N, pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 6.6
-
 Urea strain AI3, pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 10.8
-
 Hydroxyurea strain AIU1N, pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 41.3
-
 Urea strain AIU1N pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 50
-
 Cyanate strain OUCH4, pH 7.2 and 37°C Pseudomonas aeruginosa
3.5.1.4 337
-
 Hydroxyurea strain OUCH4, pH 7.2 and 37°C Pseudomonas aeruginosa