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Literature summary extracted from

  • Brown, P.R.; Smyth, M.J.; Clarke, P.H.; Rosemeyer, M.A.
    The subunit structure of the aliphatic amidase from Pseudomonas aeruginosa (1973), Eur. J. Biochem., 34, 177-187.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 35000
-
 6 * 35000, SDS-PAGE Pseudomonas aeruginosa
3.5.1.4 200000
-
 gel filtration and sedimentation equilibrium analysis, sedimentation coeffient 10.6 S Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 RCONH2 + H2O Pseudomonas aeruginosa
-
 RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O Pseudomonas aeruginosa PAC 142 (L10R)
-
 RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O Pseudomonas aeruginosa PAC 111 (C11)
-
 RCOOH + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Pseudomonas aeruginosa
-
-
-
3.5.1.4 Pseudomonas aeruginosa PAC 111 (C11)
-
-
-
3.5.1.4 Pseudomonas aeruginosa PAC 142 (L10R)
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.1.4 protein was oxidized in performic acid at -10°C for 4 h Pseudomonas aeruginosa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 streptomycin sulfate treatment, a heat treatment, ammonium sulfate fractionation and column chromatography on DEAE-Sephadex , yield 30%, purification was 19fold Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 RCONH2 + H2O
-
 Pseudomonas aeruginosa RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O
-
 Pseudomonas aeruginosa PAC 142 (L10R) RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O
-
 Pseudomonas aeruginosa PAC 111 (C11) RCOOH + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 hexamer 6 * 35000, SDS-PAGE Pseudomonas aeruginosa