Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rajagopal, R.; Tsurusaki, K.I.; Kannangara, G.; Kuraishi, S.; Sakurai, N.
    Natural ccurrence of indoleacetamide and amidohydrolase activity in etiolated aseptically-grown squash seedlings (1994), Plant Cell Physiol., 35, 329-339.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.08
-
 Indole-3-acetamide
-
 Cucurbita maxima
3.5.1.4 1.2
-
 Indole-3-acetamide
-
 Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 1-naphthaleneacetamide + H2O Cucurbita maxima
-
 1-naphthaleneacetic acid + NH3
-
 ?
3.5.1.4 2 indole-3-acetonitrile + 3 H2O Cucurbita maxima
-
 indole-3-acetamide + indole-3-acetic acid + NH3
-
 ?
3.5.1.4 indole-3-acetamide + H2O Cucurbita maxima
-
 indole-3-acetic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Agrobacterium tumefaciens
-
 Crown gall
-
3.5.1.4 Cucurbita maxima
-
 Duch, tissues of aseptically grown squash
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 enriched 48fold by fractional precipitation with ammonium sulfate, Sephadex gel filtration and anion exchange chromatography Cucurbita maxima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.4 root of raised seedling Cucurbita maxima
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 0.000028
-
 substrate indoleacetonitrile at 30°C, activity after purification Cucurbita maxima
3.5.1.4 0.000031
-
 substrate indole-3-acetamide at 30°C, activity after purification Cucurbita maxima

Storage Stability

EC Number Storage Stability Organism
3.5.1.4 stable to freezing Cucurbita maxima
3.5.1.4 unstable even at 4°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 1-naphthaleneacetamide + H2O
-
 Cucurbita maxima 1-naphthaleneacetic acid + NH3
-
 ?
3.5.1.4 2 indole-3-acetonitrile + 3 H2O
-
 Cucurbita maxima indole-3-acetamide + indole-3-acetic acid + NH3
-
 ?
3.5.1.4 indole-3-acetamide + H2O
-
 Cucurbita maxima indole-3-acetic acid + NH3
-
 ?
3.5.1.4 indole-3-acetamide + H2O
-
 Agrobacterium tumefaciens indole-3-acetate + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 45
-
-
 Cucurbita maxima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.4 30 37
-
 Agrobacterium tumefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 7 7.5
-
 Cucurbita maxima
3.5.1.4 8.5 9.5
-
 Agrobacterium tumefaciens