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Literature summary extracted from

  • Hayashi, T.; Yamamoto, K.; Matsuo, A.; Otsubo, K.; Muramatsu, S.; Matsuda, A.; Komatsu, K.I.
    Characterization and cloning of an enantioselective amidase from Comamonas acidovorans KPO-2771-4 (1997), J. Ferment. Bioeng., 83, 139-145.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.1.4 medicine therapy Delftia acidovorans
3.5.1.4 pharmacology nonsteroid anti-inflammatory drugs known as profens, chiral drug Delftia acidovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 CaCl2 relative activity 91.7%, incubated for 10 min Delftia acidovorans
3.5.1.4 Cu2+ relative activity 0%, incubated for 10 min Delftia acidovorans
3.5.1.4 Cu2+
-
 Escherichia coli
3.5.1.4 Hg2+ relative activity 91.7%, incubated for 10 min Delftia acidovorans
3.5.1.4 Hg2+
-
 Escherichia coli
3.5.1.4 iodoacetate no inhibition, incubated for 10 min Delftia acidovorans
3.5.1.4 Mg2+ relative activity 91.7%, incubated for 10 min Delftia acidovorans
3.5.1.4 MnCl2 relative activity 94.4%, incubated for 10 min Delftia acidovorans
3.5.1.4 p-chloromercuribenzoate relative activity 36.1%, incubated for 10 min Delftia acidovorans
3.5.1.4 Zn2+ relative activity 94.4%, incubated for 10 min Delftia acidovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.4 additional information enzyme has no metal ion requirement Delftia acidovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 55000
-
 gel filtration Delftia acidovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 2-arylpropionic amide + H2O Delftia acidovorans enantioselective S-amidase (S)-2-arylpropionic acid + NH3
-
 ?
3.5.1.4 2-arylpropionic amide + H2O Delftia acidovorans KPO-2771-4 enantioselective S-amidase (S)-2-arylpropionic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Delftia acidovorans
-
-
-
3.5.1.4 Delftia acidovorans KPO-2771-4
-
-
-
3.5.1.4 Escherichia coli
-
 harboring pALJ30 and pALJ20
-
3.5.1.4 Escherichia coli JM109
-
 harboring pALJ30 and pALJ20
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 0.383
-
 strain JM 109 harboring pKPO2(6) Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 2-arylpropionic amide + H2O enantioselective S-amidase Delftia acidovorans (S)-2-arylpropionic acid + NH3
-
 ?
3.5.1.4 2-arylpropionic amide + H2O enantioselective S-amidase Delftia acidovorans KPO-2771-4 (S)-2-arylpropionic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 monomer 1 * 55000, gel filtration Delftia acidovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 35
-
 activity decreases above 40°C Delftia acidovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 9
-
-
 Delftia acidovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 8.5 10
-
 Delftia acidovorans