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Literature summary extracted from

  • Nawaz, M.S.; Khan, A.A.; Bhattacharayya, D.; Siitonen, P.H.; Cerniglia, C.E.
    Physical, biochemical, and immunological characterization of a thermostable amidase from Klebsiella pneumoniae NCTR 1 (1996), J. Bacteriol., 178, 2397-2401.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.4 synthesis detoxification of acrylamide and as biocatalysts for the industrial production of ammonium acrylate and acrylic acid Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 EDTA 10 mM, loss of amidase activity of less than 5% Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 1.7
-
 Acrylamide
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.4 Cobalt 0.33 mol of cobalt per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis Klebsiella pneumoniae
3.5.1.4 Iron 0.33 mol of iron per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 62000
-
 1 * 62000, gel filtration Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 acetone precipitation, DEAE-Sepharose, ammonium sulfate precipitation, yield 10% Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 2.69
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acrylamide + H2O
-
 Klebsiella pneumoniae acrylate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 monomer 1 * 62000, gel filtration Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 65
-
 at pH 7.0 Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 7
-
-
 Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 5 8.5
-
 Klebsiella pneumoniae