Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kobayashi, M.; Komeda, H.; Nagasawa, T.; Nishiyama, M.; Hoinouchi, Sueharu.; Beppu, T.; Yamada, H.; Shimizu.
    Amidase couple with low-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1 (1993), Eur. J. Biochem., 217, 327-336.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.4 synthesis acrylamide production Rhodococcus rhodochrous

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 Ag+ 22% of origin activity Escherichia coli
3.5.1.4 Cu2+ 58% of origin activity Escherichia coli
3.5.1.4 Fe2+ 58% of origin activity Escherichia coli
3.5.1.4 Hg2+ 7% of the original activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.15
-
 Benzamide
-
 Escherichia coli
3.5.1.4 0.48
-
 Propionamide
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 54630
-
-
 Rhodococcus rhodochrous
3.5.1.4 55000
-
 2 * 55000 Escherichia coli
3.5.1.4 110000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 (R)-(+)-2-chloropropionamide + H2O Escherichia coli
-
 (+)-2-chloropropionic acid + NH3
-
 ?
3.5.1.4 (R)-(+)-2-chloropropionamide + H2O Escherichia coli JM109
-
 (+)-2-chloropropionic acid + NH3
-
 ?
3.5.1.4 (S)-(-)-2-chloropropionamide + H2O Escherichia coli
-
 (-)-2-chloropropionic acid + NH3
-
 ?
3.5.1.4 (S)-(-)-2-chloropropionamide + H2O Escherichia coli JM109
-
 (-)-2-chloropropionic acid + NH3
-
 ?
3.5.1.4 benzamide + H2O Escherichia coli
-
 benzoic acid + NH3
-
 ?
3.5.1.4 benzamide + H2O Escherichia coli JM109
-
 benzoic acid + NH3
-
 ?
3.5.1.4 RCONH2 + H2O Escherichia coli
-
 RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O Rhodococcus rhodochrous
-
 RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O Escherichia coli JM109
-
 RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O Rhodococcus rhodochrous J1
-
 RCOOH + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Escherichia coli
-
 harboring pALJ30 and pALJ20
-
3.5.1.4 Escherichia coli JM109
-
 harboring pALJ30 and pALJ20
-
3.5.1.4 Rhodococcus rhodochrous
-
-
-
3.5.1.4 Rhodococcus rhodochrous J1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 preparation of cell-free extracts by sonification, DEAE-Sephacel column chromatography, ammonium sulfate fraction, Mono-Q column chromatography, Superose 12 column chromatography, yield 30.4% Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 0.00243
-
 harboring pALJ20, substrate cyclohexanecarboxamide with cobalt Escherichia coli
3.5.1.4 0.0135
-
 substrate cyclohexanecarboxamide with cobalt Rhodococcus rhodochrous
3.5.1.4 0.468
-
 harboring pALJ30, substrate cyclohexanecarboxamide with cobalt Escherichia coli
3.5.1.4 12.2
-
 substrate benzamide Escherichia coli
3.5.1.4 12.2
-
 transformant, substrate benzamide Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 (R)-(+)-2-chloropropionamide + H2O
-
 Escherichia coli (+)-2-chloropropionic acid + NH3
-
 ?
3.5.1.4 (R)-(+)-2-chloropropionamide + H2O
-
 Escherichia coli JM109 (+)-2-chloropropionic acid + NH3
-
 ?
3.5.1.4 (S)-(-)-2-chloropropionamide + H2O
-
 Escherichia coli (-)-2-chloropropionic acid + NH3
-
 ?
3.5.1.4 (S)-(-)-2-chloropropionamide + H2O
-
 Escherichia coli JM109 (-)-2-chloropropionic acid + NH3
-
 ?
3.5.1.4 benzamide + H2O
-
 Escherichia coli benzoic acid + NH3
-
 ?
3.5.1.4 benzamide + H2O
-
 Escherichia coli JM109 benzoic acid + NH3
-
 ?
3.5.1.4 propionamide + H2O
-
 Escherichia coli propionate + NH3
-
 ?
3.5.1.4 propionamide + H2O
-
 Escherichia coli JM109 propionate + NH3
-
 ?
3.5.1.4 RCONH2 + H2O
-
 Escherichia coli RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O
-
 Rhodococcus rhodochrous RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O
-
 Escherichia coli JM109 RCOOH + NH3
-
 ?
3.5.1.4 RCONH2 + H2O
-
 Rhodococcus rhodochrous J1 RCOOH + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 dimer 2 * 55000 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 55
-
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 7.9
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 6.7 10
-
 Escherichia coli