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Literature summary extracted from

  • Soong, C.L.; Ogawa, J.; Shimizu, S.
    A novel amidase, half-amidase, for half-amide hydrolysis involved in the metabolism of cyclic imides (2000), Appl. Environ. Microbiol., 66, 1947-1952.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.4 synthesis
-
 Blastobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 Ag+ inactivates enzyme activity by 76% Blastobacter sp.
3.5.1.4 Cd2+ inactivates enzyme activity by 79% Blastobacter sp.
3.5.1.4 Cu2+ inactivates enzyme activity by 90% Blastobacter sp.
3.5.1.4 Hg2+ inactivates enzyme activity by 99% Blastobacter sp.
3.5.1.4 succinate
-
 Blastobacter sp.
3.5.1.4 sulfhydyl reagents
-
 Blastobacter sp.
3.5.1.4 Zn2+ inactivates enzyme activity by 88% Blastobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 2.8
-
 glutaramic acid
-
 Blastobacter sp.
3.5.1.4 6.2
-
 succinamic acid
-
 Blastobacter sp.
3.5.1.4 6.5
-
 Benzamide
-
 Blastobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 46000
-
 1 * 48000, gel filtration and 1 * 46000, SDS-PAGE Blastobacter sp.
3.5.1.4 48000
-
 1 * 48000, gel filtration and 1 * 46000, SDS-PAGE Blastobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 benzamide Blastobacter sp.
-
 benzoic acid + NH3
-
 ?
3.5.1.4 glutaramic acid + H2O Blastobacter sp.
-
 glutaric acid + NH3
-
 ?
3.5.1.4 monoamidated dicarboxylate + H2O Blastobacter sp.
-
 dicarboxylate + ammonia
-
 ?
3.5.1.4 succinamic acid + H2O Blastobacter sp.
-
 succinic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Blastobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 cell extract purified by DEAE-Sephacel column, concentrated by ultrafiltration, phenyl-Sepharose column, Sephacryl S-200 column, Mono Q HR 5/5 and Mono Q PC 1.6/5 column Blastobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 benzamide
-
 Blastobacter sp. benzoic acid + NH3
-
 ?
3.5.1.4 glutaramic acid + H2O
-
 Blastobacter sp. glutaric acid + NH3
-
 ?
3.5.1.4 monoamidated dicarboxylate + H2O
-
 Blastobacter sp. dicarboxylate + ammonia
-
 ?
3.5.1.4 succinamic acid + H2O
-
 Blastobacter sp. succinic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 monomer 1 * 48000, gel filtration and 1 * 46000, SDS-PAGE Blastobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.4 half-amidase for half-amide hydrolysis Blastobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 35
-
-
 Blastobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 0.00217
-
 Benzamide
-
 Blastobacter sp.
3.5.1.4 0.0372
-
 glutaramic acid
-
 Blastobacter sp.
3.5.1.4 0.096
-
 succinamic acid
-
 Blastobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.4 8 10 various buffer systems, 80% of the activity retained at 30°C for 30 min Blastobacter sp.