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Literature summary extracted from

  • Silman, N.J.; Carver, A.M.; Jones, C.W.
    Directed evolution of amidase in Methylophilus methylotrophus; purification and properties of amidases from wild-type and mutants strains (1991), J. Gen. Microbiol., 137, 169-178.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 DTNB 15-18% inhibition of wild-type, MM6 and MM8, 35% inhibition of the MM15 activity Methylophilus methylotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 1.2
-
 acetamide mutant strain MM8 Methylophilus methylotrophus
3.5.1.4 2
-
 Acrylamide mutant strain MM8 Methylophilus methylotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 38000
-
 4 * 38000 Methylophilus methylotrophus
3.5.1.4 38400
-
 6 * 38400 Pseudomonas aeruginosa
3.5.1.4 40000
-
 8 * 40000 Arthrobacter sp.
3.5.1.4 155000
-
 sedimentation-equilibrium ultracentrifugation Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 acetamide + H2O Methylophilus methylotrophus
-
 acetic acid + NH3
-
 ?
3.5.1.4 acylamide + H2O Methylophilus methylotrophus
-
 carboxylic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Arthrobacter sp.
-
-
-
3.5.1.4 Methylophilus methylotrophus
-
 wild-type is strain MM6, mutant strains are MM8 and MM15
-
3.5.1.4 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 passage with French pressure cell, cell-free extract purified and concentrated by using anion-exchange and gel filtration, judged by SDS-PAGE and scanning densiometry Methylophilus methylotrophus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 1.51
-
 mutant strain MM15 measured with acrylamide as substrate, growth conditions is continuous Methylophilus methylotrophus
3.5.1.4 2.1
-
 mutant strain MM15 measured with acetamide as substrate Methylophilus methylotrophus
3.5.1.4 3.59
-
 mutant strain MM15 measured with acrylamide as substrate, growth conditions is fed-batch Methylophilus methylotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acetamide + H2O
-
 Methylophilus methylotrophus acetic acid + NH3
-
 ?
3.5.1.4 acrylamide + H2O
-
 Methylophilus methylotrophus acrylate + NH3
-
 ?
3.5.1.4 acylamide + H2O
-
 Methylophilus methylotrophus carboxylic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 hexamer 6 * 38400 Pseudomonas aeruginosa
3.5.1.4 octamer 8 * 40000 Arthrobacter sp.
3.5.1.4 tetramer 4 * 38000 Methylophilus methylotrophus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 2.13
-
 acetamide
-
 Methylophilus methylotrophus