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Literature summary extracted from

  • Hersh, L.B.; Tsai, L.; Stadtman, E.R.
    The enzymatic synthesis of 5-hydroxy-N-methylpyroglutamic acid (1969), J. Biol. Chem., 244, 4677-4683.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.36 additional information not inhibited by triethanolamine, 2-methylimidazole, diphosphate and Tricine buffer Pseudomonas sp.
3.5.1.36 Tris-HCl
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.36 38
-
 alpha-ketoglutarate
-
 Pseudomonas sp.
3.5.1.36 46
-
 methylamine
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.36 alpha-ketoglutarate + methylamine Pseudomonas sp.
-
 N-methyl-2-oxoglutaramate + H2O
-
 ?
3.5.1.36 alpha-ketoglutarate + methylamine Pseudomonas sp. MA / M.A. / ATCC 23819
-
 N-methyl-2-oxoglutaramate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.36 Pseudomonas sp.
-
-
-
3.5.1.36 Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.36 0.98
-
 after partial purification Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.36 alpha-ketoglutarate + methylamine
-
 Pseudomonas sp. N-methyl-2-oxoglutaramate + H2O
-
 ?
3.5.1.36 alpha-ketoglutarate + methylamine
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 N-methyl-2-oxoglutaramate + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.36 additional information
-
 after heated 12 min to 50°C the activity of enzyme is 12%. Untreated enzyme stable Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.36 8
-
 enzymatic reaction exhibits a pH maximum of about 8 Pseudomonas sp.