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Literature summary extracted from

  • Tarentino, A.I.; Maley, F.
    The purification and properties of a beta-aspartyl N-acetylglucosylamine amidohydrolase from hen oviduct (1969), Arch. Biochem. Biophys., 130, 295-303.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.26 5-Diazo-4-oxo-L-norvaline non-competitive inhibition, Ki: 0.0095 mM, protection by its natural substrate Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.26 0.05
-
 1-L-beta-aspartamido-(2-acetamido)-1,2-dideoxy-beta-D-glucose
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.26 101000
-
 gel filtration, sucrose density gradient centrifugation Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.26 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.26 near homogeneity Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.26 oviduct
-
 Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.26 -20°C, 3 months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.26 2-acetamido-1-beta-(L-aspartamido)-1,2-dideoxy-beta-D-glucose + H2O 2 stages of hydrolysis Gallus gallus 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate non-enzymatic degradation of 1-amino-N-acetylglucosamine to NH3 + N-acetyl-beta-D-glucosamine ir
3.5.1.26 ovalbumin glycopeptide + H2O intermediate activity Gallus gallus L-aspartate + ?
-
 ?
3.5.1.26 ribonuclease B glycopeptide + H2O poor substrate, more activity with the pure glycopeptide Gallus gallus L-aspartate + ?
-
 ?
3.5.1.26 sialo-transferrin + H2O intermediate activity Gallus gallus L-aspartate + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.26 5.1
-
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.26 6.5 8.5 only gradual decrease in activity within Gallus gallus