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Literature summary extracted from

  • Tikkanen, R.; Riikonen, A.; Oinonen, C.; Rouvinen, J.; Peltonen, L.
    Functional analyses of active site residues of human lysosomal aspartylglucosaminidase: implications for catalytic mechanism and autocatalytic activation (1996), EMBO J., 15, 2954-2960.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.26 expression in COS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.26 R234A nearly complete decrease of activity, misprocessing of precursor in ER Homo sapiens
3.5.1.26 R234L nearly complete decrease of activity, misprocessing of precursor in ER Homo sapiens
3.5.1.26 R234Q nearly complete decrease of activity, misprocessing of precursor in ER Homo sapiens
3.5.1.26 T224A nearly complete decrease of activity Homo sapiens
3.5.1.26 T224S nearly complete decrease of activity Homo sapiens
3.5.1.26 T257A nearly complete decrease of activity Homo sapiens
3.5.1.26 T257A nearly complete decrease of activity, misprocessing of precursor in ER Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.26 2.5
-
 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.26 lysosome
-
 Homo sapiens 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.26 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O Homo sapiens involved in degradation of glycoproteins 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.26 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.26 to homogeneity, chromatography techniques Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.26 leukocyte
-
 Homo sapiens
-
3.5.1.26 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.26 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O involved in degradation of glycoproteins Homo sapiens 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 ?