BRENDA - Enzyme Database

Architectures of class-defining and specific domains of glutamyl-tRNA synthetase

Nureki, O.; Vassylyev, D.G.; Katayanagi, K.; Shimizu, T.; Sekine, S.i.; Kigawa, T.; Miyazawa, T.; Yokoyama, S.; Morikawa, K.; Science 267, 1958-1965 (1995)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
6.1.1.17
architectures of class-defining and specific domains
Thermus thermophilus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
additional information
mutant enzymes with higher Km and lower turnover numbers
Thermus thermophilus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
Km-values of mutant enzymes
Thermus thermophilus
6.1.1.17
0.00273
-
tRNAGlu
wild-type enzyme
Thermus thermophilus
6.1.1.17
0.023
-
ATP
wild-type enzyme
Thermus thermophilus
6.1.1.17
0.12
-
L-Glu
wild-type
Thermus thermophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Thermus thermophilus
-
wild-type and mutant enzymes
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
209
Thermus thermophilus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
209
Thermus thermophilus
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
turnover numbers of wild-type and mutant enzymes
Thermus thermophilus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
6.1.1.17
architectures of class-defining and specific domains
Thermus thermophilus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
additional information
mutant enzymes with higher Km and lower turnover numbers
Thermus thermophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
Km-values of mutant enzymes
Thermus thermophilus
6.1.1.17
0.00273
-
tRNAGlu
wild-type enzyme
Thermus thermophilus
6.1.1.17
0.023
-
ATP
wild-type enzyme
Thermus thermophilus
6.1.1.17
0.12
-
L-Glu
wild-type
Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
209
Thermus thermophilus
AMP + diphosphate + L-glutamyl-tRNAGlu
-
209
Thermus thermophilus
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
turnover numbers of wild-type and mutant enzymes
Thermus thermophilus