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Literature summary extracted from
- Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli (1995), J. Bacteriol., 3, 1292-1298.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.10 | methionine | - |
Escherichia coli |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.10 | purU gene cloned into overexpression vector pT7-7, pT7-PU1 tranformed into Escherichia coli BL21(DE3) | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.10 | glycine | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.10 | 0.049 | - |
10-formyltetrahydrofolate | 2.0 mM methionine added | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.10 | 31800 | - |
calculated from the sequence of the cloned gene | Escherichia coli |
| 3.5.1.10 | 32000 | - |
6 * 32000, SDS-PAGE | Escherichia coli |
| 3.5.1.10 | 190000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.10 | Escherichia coli | P37051 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.10 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.10 | 0.174 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.10 | 10-formyltetrahydrofolate + H2O | - |
Escherichia coli | formate + tetrahydrofolate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.10 | hexamer | 6 * 32000, SDS-PAGE | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.10 | formyl-FH4 hydrolase | - |
Escherichia coli |
| 3.5.1.10 | formyltetrahydrofolate hydrolase | - |
Escherichia coli |
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