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Literature summary extracted from

  • Manna, S.; Sinha, A.; Sadhukhan, R.; Chakrabarty, S.L.
    Purification, characterization and antitumor activity of L-asparaginase isolated from Pseudomonas stutzeri MB-405 (1995), Curr. Microbiol., 30, 291-298.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 2-mercaptoethanol enhances activity Pseudomonas stutzeri
3.5.1.1 dithiothreitol enhances activity Pseudomonas stutzeri
3.5.1.1 EDTA enhances activity Pseudomonas stutzeri
3.5.1.1 glutathione enhances activity Pseudomonas stutzeri
3.5.1.1 GSH enhances activity Pseudomonas stutzeri
3.5.1.1 L-Cys enhances activity Pseudomonas stutzeri
3.5.1.1 L-His enhances activity Pseudomonas stutzeri

Application

EC Number Application Comment Organism
3.5.1.1 medicine enzyme has antitumor activity Pseudomonas stutzeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 5-Diazo-4-oxo-L-norvaline
-
 Pseudomonas stutzeri
3.5.1.1 Ca2+
-
 Pseudomonas stutzeri
3.5.1.1 Cd2+
-
 Pseudomonas stutzeri
3.5.1.1 Cu2+
-
 Pseudomonas stutzeri
3.5.1.1 Fe3+
-
 Pseudomonas stutzeri
3.5.1.1 Hg2+
-
 Pseudomonas stutzeri
3.5.1.1 iodoacetamide
-
 Pseudomonas stutzeri
3.5.1.1 Mg2+
-
 Pseudomonas stutzeri
3.5.1.1 Mn2+
-
 Pseudomonas stutzeri
3.5.1.1 Ni2+
-
 Pseudomonas stutzeri
3.5.1.1 PCMB
-
 Pseudomonas stutzeri
3.5.1.1 SDS
-
 Pseudomonas stutzeri
3.5.1.1 Urea
-
 Pseudomonas stutzeri
3.5.1.1 Zn2+
-
 Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.145
-
 L-Asn
-
 Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 K+ activates Pseudomonas stutzeri
3.5.1.1 Mg2+ stimulates Pseudomonas stutzeri
3.5.1.1 Na+ activates Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 33000
-
 1 * 33000, SDS-PAGE Pseudomonas stutzeri
3.5.1.1 34000
-
 gel filtration Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pseudomonas stutzeri
-
 MB-405
-
3.5.1.1 Pseudomonas stutzeri MB-405
-
 MB-405
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 732.3
-
-
 Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 beta-cyano-L-Ala + H2O 9.5% of the activity with L-Asn Pseudomonas stutzeri ?
-
 ?
3.5.1.1 beta-cyano-L-Ala + H2O 9.5% of the activity with L-Asn Pseudomonas stutzeri MB-405 ?
-
 ?
3.5.1.1 L-Asn + H2O
-
 Pseudomonas stutzeri L-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Pseudomonas stutzeri MB-405 L-Asp + NH3
-
 ?
3.5.1.1 L-aspartic acid beta-hydroxamate + H2O 11% of the activity with L-Asn Pseudomonas stutzeri L-Asp + hydroxylamine
-
 ?
3.5.1.1 L-aspartic acid beta-hydroxamate + H2O 11% of the activity with L-Asn Pseudomonas stutzeri MB-405 L-Asp + hydroxylamine
-
 ?
3.5.1.1 L-Gln + H2O
-
 Pseudomonas stutzeri L-Glu + NH3
-
 ?
3.5.1.1 L-Gln + H2O
-
 Pseudomonas stutzeri MB-405 L-Glu + NH3
-
 ?
3.5.1.1 additional information enzyme has antitumor activity Pseudomonas stutzeri ?
-
 ?
3.5.1.1 additional information enzyme has antitumor activity Pseudomonas stutzeri MB-405 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 monomer 1 * 33000, SDS-PAGE Pseudomonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
-
 Pseudomonas stutzeri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 30 55 30°C: about 60% of maximal activity, 55°C: about 55% of maximal activity Pseudomonas stutzeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 40
-
 60 min, no significant loss of activity Pseudomonas stutzeri
3.5.1.1 60
-
 half-life: 25 min Pseudomonas stutzeri
3.5.1.1 65
-
 half-life: 10 min Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 9
-
-
 Pseudomonas stutzeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 6.5 10.5 pH 6.5: about 45% of maximal activity, pH 10.5: about 65% of maximal activity Pseudomonas stutzeri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.1 7.5 9.5 maximal stability in the range Pseudomonas stutzeri