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Literature summary extracted from

  • Tosa, T.; Sano, R.; Yamamoto, K.; Nakamura, M.; Chibata, I.
    L-Asparaginase from Proteus vulgaris. Purification, crystallization, and enzymic properties (1972), Biochemistry, 11, 217-222.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.1
-
Proteus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.026
-
L-asparagine
-
Proteus vulgaris
3.5.1.1 0.43
-
D-Asn
-
Proteus vulgaris
3.5.1.1 5
-
L-Gln
-
Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Proteus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
Proteus vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 300
-
-
Proteus vulgaris

Storage Stability

EC Number Storage Stability Organism
3.5.1.1 -20°C, stable for 1 month Proteus vulgaris
3.5.1.1 5°C, as suspension in 3.5 M ammonium sulfate, stable for 1 year Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 D-Asn + H2O 10% of the activity with L-Asn Proteus vulgaris D-Asp + NH3
-
?
3.5.1.1 D-aspartic acid beta-hydroxamate + H2O 59% of the activity with L-Asn Proteus vulgaris D-Asp + hydroxylamine
-
?
3.5.1.1 L-Asn + H2O
-
Proteus vulgaris L-Asp + NH3
-
?
3.5.1.1 L-aspartic acid beta-hydrazide + H2O 2% of the activity with L-Asn Proteus vulgaris L-Asp + hydroxylamine
-
?
3.5.1.1 L-aspartic acid beta-hydroxamate + H2O 59% of the activity with L-Asn Proteus vulgaris L-Asp + hydroxylamine
-
?
3.5.1.1 L-Gln + H2O 2% of the activity with L-Asn Proteus vulgaris L-Glu + NH3
-
?
3.5.1.1 L-glutamic acid gamma-hydroxamate + H2O 1% of the activity with L-Asn Proteus vulgaris L-Glu + hydroxylamine
-
?
3.5.1.1 Nalpha-methyl-L-Asn + H2O 26% of the activity with L-Asn Proteus vulgaris Nalpha-methyl-L-Asp + NH3
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 57
-
-
Proteus vulgaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 32 62 32°C: about 60% of maximal activity, 62°C: about 85% pf maximal activity Proteus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 7 8
-
Proteus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 4 11 pH 4.0: about 50% of maximal activity, pH 11.0: about 75% of maximal activity Proteus vulgaris