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Literature summary extracted from

  • Lea, P.J.; Fowden, L.; Miflin, B.J.
    The purification and properties of asparaginase from Lupinus species (1978), Phytochemistry, 17, 217-222.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 PCMB slight stimulation Lupinus polyphyllus
3.5.1.1 Tartrate stimulation Lupinus polyphyllus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 2-amino-5-chloro-4-oxo pentanoic acid
-
 Lupinus polyphyllus
3.5.1.1 3-Cyano-L-Ala
-
 Lupinus polyphyllus
3.5.1.1 4-methylene-L-glutamine
-
 Lupinus polyphyllus
3.5.1.1 5-Bromo-4-oxo-L-norvaline
-
 Lupinus polyphyllus
3.5.1.1 Br-
-
 Lupinus polyphyllus
3.5.1.1 Cd2+
-
 Lupinus polyphyllus
3.5.1.1 Co2+
-
 Lupinus polyphyllus
3.5.1.1 Cu2+
-
 Lupinus polyphyllus
3.5.1.1 Dichromate
-
 Lupinus polyphyllus
3.5.1.1 DL-aspartate 3-hydroxamate
-
 Lupinus polyphyllus
3.5.1.1 Fe2+
-
 Lupinus polyphyllus
3.5.1.1 Mg2+
-
 Lupinus polyphyllus
3.5.1.1 N4-hydroxyethyl-L-asparagine
-
 Lupinus polyphyllus
3.5.1.1 Ni2+
-
 Lupinus polyphyllus
3.5.1.1 Pb4+
-
 Lupinus polyphyllus
3.5.1.1 Sn2+
-
 Lupinus polyphyllus
3.5.1.1 sulfate
-
 Lupinus polyphyllus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 12.2
-
 L-asparagine
-
 Lupinus polyphyllus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Ca2+ slight stimulation Lupinus polyphyllus
3.5.1.1 diphosphate stimulation Lupinus polyphyllus
3.5.1.1 HPO42- stimulation Lupinus polyphyllus
3.5.1.1 K3Fe(CN)6 stimulation Lupinus polyphyllus
3.5.1.1 Na+ slight stimulation Lupinus polyphyllus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 38000
-
 x * 38000, SDS-PAGE Lupinus polyphyllus
3.5.1.1 72000
-
 sucrose density gradient centrifugation Lupinus polyphyllus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Lupinus polyphyllus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Lupinus polyphyllus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 seed
-
 Lupinus polyphyllus
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.1 -20°C, less than 10% loss of activity after 3 months Lupinus polyphyllus
3.5.1.1 4°C, stable for up to 4 days Lupinus polyphyllus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 DL-aspartyl hydroxamate + H2O
-
 Lupinus polyphyllus DL-Asp + hydroxylamine
-
 ?
3.5.1.1 L-Asn + H2O
-
 Lupinus polyphyllus L-Asp + NH3
-
 ?
3.5.1.1 L-Gln + H2O no activity Lupinus polyphyllus L-Glu + NH3
-
 ?
3.5.1.1 N4-ethyl-L-asparagine 12% of the activity with L-Asn Lupinus polyphyllus L-Asp + propylamine
-
 ?
3.5.1.1 N4-methoxy-L-asparagine + H2O 154% of the activity with L-Asn Lupinus polyphyllus L-Asp + O-methylhydroxylamine
-
 ?
3.5.1.1 N4-methyl-L-asparagine 12% of the activity with L-Asn Lupinus polyphyllus L-Asp + methylamine
-
 ?
3.5.1.1 threo-3-hydroxy-L-asparagine + H2O 36% of the activity with L-Asn Lupinus polyphyllus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 ? x * 38000, SDS-PAGE Lupinus polyphyllus