Substrate specificity and stereospecifity of limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis DCL14; an enzyme showing sequential and enantioconvergent substrate conversion
Van der Werf, M.J.; Orru, R.V.A.; Overkamp, K.M.; Swarts, H.J.; Ospiran, I.; Steinreiber, A.; de Bont, J.A.M.; Faber, K.; Appl. Microbiol. Biotechnol. 52, 380-385 (1999)
No PubMed abstract available
Data extracted from this reference:
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.3.2.8
cytoplasm
-
Rhodococcus erythropolis
5737
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.3.2.8
(1R,2S,4R)-limonene-1,2-epoxide + H2O
Rhodococcus erythropolis
-
(1S,2S,4R)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
Rhodococcus erythropolis
?
3.3.2.8
(1R,2S,4S)-limonene-1,2-epoxide + H2O
Rhodococcus erythropolis
-
(1R,2R,4S)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
Rhodococcus erythropolis
?
3.3.2.8
(1S,2R,4R)-limonene-1,2-epoxide + H2O
Rhodococcus erythropolis
-
(1S,2S,4R)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
Rhodococcus erythropolis
?
3.3.2.8
(1S2R4S)-limonene-1,2-epoxide + H2O
Rhodococcus erythropolis
-
(1R,2R,4S)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
Rhodococcus erythropolis
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.3.2.8
Rhodococcus erythropolis
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.3.2.8
1,2-epoxymenth-8-ene + H2O = menth-8-ene-1,2-diol
catalyzes simultaneous sequential and enantioconvergent epoxide conversion
Rhodococcus erythropolis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.3.2.8
85.1
-
with (1R,2S,4R)-limonene-1,2-epoxide as substrate
Rhodococcus erythropolis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.3.2.8
(1R,2S,4R)-limonene-1,2-epoxide + H2O
-
208936
Rhodococcus erythropolis
(1S,2S,4R)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
208936
Rhodococcus erythropolis
?
3.3.2.8
(1R,2S,4S)-limonene-1,2-epoxide + H2O
-
208936
Rhodococcus erythropolis
(1R,2R,4S)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
208936
Rhodococcus erythropolis
?
3.3.2.8
(1S,2R,4R)-limonene-1,2-epoxide + H2O
-
208936
Rhodococcus erythropolis
(1S,2S,4R)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
208936
Rhodococcus erythropolis
?
3.3.2.8
(1S2R4S)-limonene-1,2-epoxide + H2O
-
208936
Rhodococcus erythropolis
(1R,2R,4S)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
208936
Rhodococcus erythropolis
?
3.3.2.8
1,2-epoxy-2,6-dimethyl-5-heptene + H2O
-
208936
Rhodococcus erythropolis
(2S)-2,6-dimethyl-5-hepten-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
1,2-epoxy-2-methyl-6-heptene + H2O
-
208936
Rhodococcus erythropolis
(2S)-2-methyl-6-heptene-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
1,2-epoxy-2-methylheptane + H2O
-
208936
Rhodococcus erythropolis
(2S)-2-methylheptane-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
1,2-epoxy-3-benzyl-2-methylpropane + H2O
-
208936
Rhodococcus erythropolis
(2S)-3-benzyl-2-methylpropane-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
1-methylcyclohexene oxide + H2O
-
208936
Rhodococcus erythropolis
(1S,2S)-1-methylcyclohexane-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
cyclohexene oxide + H2O
-
208936
Rhodococcus erythropolis
(1S,2S)-trans cyclohexanediol + (1R,2R)-trans-cyclohexanediol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
indene oxide + H2O
-
208936
Rhodococcus erythropolis
indane-1,2-diol
-
208936
Rhodococcus erythropolis
?
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.3.2.8
cytoplasm
-
Rhodococcus erythropolis
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.3.2.8
(1R,2S,4R)-limonene-1,2-epoxide + H2O
Rhodococcus erythropolis
-
(1S,2S,4R)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
Rhodococcus erythropolis
?
3.3.2.8
(1R,2S,4S)-limonene-1,2-epoxide + H2O
Rhodococcus erythropolis
-
(1R,2R,4S)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
Rhodococcus erythropolis
?
3.3.2.8
(1S,2R,4R)-limonene-1,2-epoxide + H2O
Rhodococcus erythropolis
-
(1S,2S,4R)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
Rhodococcus erythropolis
?
3.3.2.8
(1S2R4S)-limonene-1,2-epoxide + H2O
Rhodococcus erythropolis
-
(1R,2R,4S)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
Rhodococcus erythropolis
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.3.2.8
85.1
-
with (1R,2S,4R)-limonene-1,2-epoxide as substrate
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.3.2.8
(1R,2S,4R)-limonene-1,2-epoxide + H2O
-
208936
Rhodococcus erythropolis
(1S,2S,4R)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
208936
Rhodococcus erythropolis
?
3.3.2.8
(1R,2S,4S)-limonene-1,2-epoxide + H2O
-
208936
Rhodococcus erythropolis
(1R,2R,4S)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
208936
Rhodococcus erythropolis
?
3.3.2.8
(1S,2R,4R)-limonene-1,2-epoxide + H2O
-
208936
Rhodococcus erythropolis
(1S,2S,4R)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
208936
Rhodococcus erythropolis
?
3.3.2.8
(1S2R4S)-limonene-1,2-epoxide + H2O
-
208936
Rhodococcus erythropolis
(1R,2R,4S)-limonene-1,2-diol
optically pure, diastereomeric excess above 99%
208936
Rhodococcus erythropolis
?
3.3.2.8
1,2-epoxy-2,6-dimethyl-5-heptene + H2O
-
208936
Rhodococcus erythropolis
(2S)-2,6-dimethyl-5-hepten-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
1,2-epoxy-2-methyl-6-heptene + H2O
-
208936
Rhodococcus erythropolis
(2S)-2-methyl-6-heptene-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
1,2-epoxy-2-methylheptane + H2O
-
208936
Rhodococcus erythropolis
(2S)-2-methylheptane-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
1,2-epoxy-3-benzyl-2-methylpropane + H2O
-
208936
Rhodococcus erythropolis
(2S)-3-benzyl-2-methylpropane-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
1-methylcyclohexene oxide + H2O
-
208936
Rhodococcus erythropolis
(1S,2S)-1-methylcyclohexane-1,2-diol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
cyclohexene oxide + H2O
-
208936
Rhodococcus erythropolis
(1S,2S)-trans cyclohexanediol + (1R,2R)-trans-cyclohexanediol
-
208936
Rhodococcus erythropolis
?
3.3.2.8
indene oxide + H2O
-
208936
Rhodococcus erythropolis
indane-1,2-diol
-
208936
Rhodococcus erythropolis
?