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Literature summary extracted from

  • Fan, J.Q.; Kadowaki, S.; Yamamoto, K.; Kumagai, H.; Tochikura, T.
    Purification and characterization of endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. (1988), Agric. Biol. Chem., 52, 1715-1723.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.97 Cu2+ 2.5 mM, 26% inhibition Alcaligenes sp.
3.2.1.97 Cys 2.5 mM, 32% inhibition Alcaligenes sp.
3.2.1.97 Hg2+ 2.5 mM, complete inhibition Alcaligenes sp.
3.2.1.97 Mn2+ 2.5 mM, 35% inhibition Alcaligenes sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.97 3.2
-
 asialo kappa-casein
-
 Alcaligenes sp.
3.2.1.97 3.7
-
 asialofetuin
-
 Alcaligenes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.97 160000
-
 gel filtration Alcaligenes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.97 additional information Alcaligenes sp. highly induced by porcine gastric mucin ?
-
 ?
3.2.1.97 additional information Alcaligenes sp. F-1906 highly induced by porcine gastric mucin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.97 Alcaligenes sp.
-
-
-
3.2.1.97 Alcaligenes sp. F-1906
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.97
-
 Alcaligenes sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.97 culture medium
-
 Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.97 2.18
-
-
 Alcaligenes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.97 asialo kappa-casein + H2O
-
 Alcaligenes sp. Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 asialo kappa-casein + H2O
-
 Alcaligenes sp. F-1906 Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 asialofetuin + H2O
-
 Alcaligenes sp. additional information Galbeta(1-3)GalNAc + ? ?
3.2.1.97 asialofetuin + H2O
-
 Alcaligenes sp. F-1906 additional information Galbeta(1-3)GalNAc + ? ?
3.2.1.97 asialoglycophorin + H2O
-
 Alcaligenes sp. Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 asialoglycophorin + H2O
-
 Alcaligenes sp. F-1906 Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 glycoprotein + H2O release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages Alcaligenes sp. Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 glycoprotein + H2O release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages Alcaligenes sp. F-1906 Galbeta(1-3)GalNAc + ?
-
 ?
3.2.1.97 additional information highly induced by porcine gastric mucin Alcaligenes sp. ?
-
 ?
3.2.1.97 additional information highly induced by porcine gastric mucin Alcaligenes sp. F-1906 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.97 monomer 1 * 160000, SDS-PAGE Alcaligenes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.97 40 45
-
 Alcaligenes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.97 35
-
 10 min, stable below Alcaligenes sp.
3.2.1.97 55
-
 10 min, complete inactivation Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.97 4.5 5
-
 Alcaligenes sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.97 4 9 about 50% of maximal activity at pH 4.0 and at pH 9.0 Alcaligenes sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.97 4.5 6.5 30°C, 1 h, stable Alcaligenes sp.