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Literature summary extracted from
- Molecular cloning of isomaltotrio-dextranase gene from Brevibacterium fuscum var. dextranlyticum strain 0407 and its expression in Escherichia coli (1999), Biosci. Biotechnol. Biochem., 63, 1582-1588.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.95 | expression in Escherichia coli | Brevibacterium fuscum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.95 | additional information | - |
additional information | Km: 0.72 mg/ml for the recombinant enzyme and 0.26 mg/ml for the native enzyme | Brevibacterium fuscum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.95 | 68000 | - |
x * 68000, SDS-PAGE | Brevibacterium fuscum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.95 | Brevibacterium fuscum | - |
strain 0407 | - |
| 3.2.1.95 | Brevibacterium fuscum | - |
var. dextranlyticum | - |
| 3.2.1.95 | Brevibacterium fuscum 407 | - |
strain 0407 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.95 | recombinant enzyme | Brevibacterium fuscum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.95 | 21.5 | - |
recombinant enzyme | Brevibacterium fuscum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.95 | dextran + H2O | - |
Brevibacterium fuscum | isomaltotriose | - |
? |  |
| 3.2.1.95 | dextran + H2O | - |
Brevibacterium fuscum 407 | isomaltotriose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.95 | ? | x * 68000, SDS-PAGE | Brevibacterium fuscum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.95 | 7.5 | - |
recombinant enzyme | Brevibacterium fuscum |
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Release 2023.1 (January 2023)
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