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Literature summary extracted from

  • Suzuki, M.; Saito, T.; Itoh, T.
    Coexistence of two kinds of 6-phospho-beta galactosidase in the cytosol of Lactobacillus gasseri JCM 1031 - purification and characterization of 6-phospho-beta-galactosidase II (1996), Biosci. Biotechnol. Biochem., 60, 708-710.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.85 2-mercaptoethanol lower effect on P-beta-gal II than P-beta-gal I Lactobacillus gasseri
3.2.1.85 Fe2+ 60% increase of activity Lactobacillus gasseri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.85 D-Glucose-6-phosphate 63% of activity Lactobacillus gasseri
3.2.1.85 iodoacetamide
-
 Lactobacillus gasseri
3.2.1.85 N-ethylmaleimide
-
 Lactobacillus gasseri
3.2.1.85 p-chloromercuribenzoic acid
-
 Lactobacillus gasseri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.85 0.76 0.8 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
 Lactobacillus gasseri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.85 Ca2+ 78% of activity at 1 mM CaCl2 Lactobacillus gasseri
3.2.1.85 Hg2+ complete inhibition at 1 mM HgCl2 Lactobacillus gasseri
3.2.1.85 Mg2+ slight inhibition at 1 mM MgCl2 Lactobacillus gasseri
3.2.1.85 Mn2+ slight inhibition at 1 mM MnCl2 Lactobacillus gasseri
3.2.1.85 Zn2+ 98.4% inhibition at 1 mM ZnSO4 Lactobacillus gasseri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O Lactobacillus gasseri
-
 D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 lactose-6-phosphate + H2O Lactobacillus gasseri JCM 1031
-
 D-glucose + beta-D-galactose-6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Lactobacillus gasseri
-
 JCM 1031
-
3.2.1.85 Lactobacillus gasseri JCM 1031
-
 JCM 1031
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.85
-
 Lactobacillus gasseri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O
-
 Lactobacillus gasseri D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 lactose-6-phosphate + H2O
-
 Lactobacillus gasseri JCM 1031 D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Lactobacillus gasseri o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Lactobacillus gasseri JCM 1031 o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.85 P-beta-gal I and II Lactobacillus gasseri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.85 30
-
 P-beta-gal II Lactobacillus gasseri
3.2.1.85 50
-
 P-beta-gal I Lactobacillus gasseri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.85 40 60 P-beta-gal II, stable below 40°C, inactivated at 60°C Lactobacillus gasseri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.85 5 5.5
-
 Lactobacillus gasseri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.85 4 9
-
 Lactobacillus gasseri