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Literature summary extracted from

  • Johnson, K.G.; McDonald, I.J.
    beta-D-Phosphogalactoside galactohydrolase from Streptococcus cremoris HP: purification and enzyme properties (1974), J. Bacteriol., 117, 667-674.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.85 dithiothreitol enhanced activiy after dialysis by addition up to 0.75 mM Lactococcus cremoris
3.2.1.85 EDTA slight stimulation to 118.2% by 2 mM Lactococcus cremoris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.85 D-galactose-6-phosphate competitive inhibition Lactococcus cremoris
3.2.1.85 p-hydroxymercuribenzoate 83.4% of control activity at 1 mM, 12.2% at 2 mM Lactococcus cremoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.85 0.59
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
 Lactococcus cremoris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.85 Li+ 82% of control activity at 2 mM Lactococcus cremoris
3.2.1.85 Mg2+ 74.5% of control activity at 2 mM Lactococcus cremoris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.85 67600
-
 gel filtration Lactococcus cremoris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O Lactococcus cremoris
-
 D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 lactose-6-phosphate + H2O Lactococcus cremoris HP
-
 D-glucose + beta-D-galactose-6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.85 Lactococcus cremoris
-
 HP
-
3.2.1.85 Lactococcus cremoris HP
-
 HP
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.85
-
 Lactococcus cremoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.85 23.8
-
 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate Lactococcus cremoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.85 lactose-6-phosphate + H2O
-
 Lactococcus cremoris D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 lactose-6-phosphate + H2O
-
 Lactococcus cremoris HP D-glucose + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Lactococcus cremoris o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?
3.2.1.85 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Lactococcus cremoris HP o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.85 beta-D-phosphogalactoside galactohydrolase
-
 Lactococcus cremoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.85 45 52 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate Lactococcus cremoris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.85 70
-
 stable up to for 5 min Lactococcus cremoris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.85 5 8 nearly constant activity Lactococcus cremoris