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Literature summary extracted from

  • Henson, C.A.; Livingston III, D.P.
    Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans (1996), Plant Physiol., 110, 639-644.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.154 additional information
-
 additional information the KM-value for neokestin is 2.8% w/v. The Km-value for 6G,6-kestotetraose is 5.6% w/v Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 43000
-
 x * 43000, SDS-PAGE Avena sativa
3.2.1.154 43000
-
 x * 43000, SDS-PAGE Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Avena sativa
-
 oat
-
3.2.1.154 Avena sativa
-
 cv. Fulghum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80
-
 Avena sativa
3.2.1.154
-
 Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 6G,6-kestotetraose + H2O 100%, chicorium inulin 11.9%, Aerobacter levan 8.5%, sucrose 0% rate of hydrolysis Avena sativa D-fructose + neokestose
-
 ?
3.2.1.80 neokestin + H2O
-
 Avena sativa D-fructose
-
 ?
3.2.1.80 neokestose + H2O
-
 Avena sativa D-fructose + sucrose
-
 ?
3.2.1.154 1,6G-kestotetraose + H2O
-
 Avena sativa 1-kestotriose + fructose
-
 ?
3.2.1.154 6G,1,6-kestopentaose + H2O
-
 Avena sativa fructose + 6G-kestotriose + ?
-
 ?
3.2.1.154 6G,6-kestotetraoase + H2O
-
 Avena sativa fructose + 6G-kestotriose
-
 ?
3.2.1.154 6G-kestotriose + H2O
-
 Avena sativa sucrose + fructose
-
 ?
3.2.1.154 inulin + H2O 11.9% of the activity with 6G,6-kestotetraoase Avena sativa ?
-
 ?
3.2.1.154 levan + H2O 8.5% of the activity with 6G,6-kestotetraoase Avena sativa ?
-
 ?
3.2.1.154 additional information no activity with sucrose Avena sativa ?
-
 ?
3.2.1.154 neokestin + H2O
-
 Avena sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.80 ? x * 43000, SDS-PAGE Avena sativa
3.2.1.154 ? x * 43000, SDS-PAGE Avena sativa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.154 6-FEH
-
 Avena sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 4.5 5.5 neokestin Avena sativa
3.2.1.154 4.5 5 hydrolysis of neokestin Avena sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.154 3.5 6.5 pH 3.5: about 70% of maximal activity, pH 6.5: about 55% of maximal activity, hydrolysis of neokestin Avena sativa