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Literature summary extracted from

  • Igarashi, T.; Yakamoto, A.; Goto, N.
    Characterization of an exo-beta-D-fructosidase from Streptococcus mutants ingbritt (1992), Microbiol. Immunol., 36, 643-647.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 Fe3+
-
 Streptococcus mutans
3.2.1.80 Hg2+
-
 Streptococcus mutans
3.2.1.80 Zn2+
-
 Streptococcus mutans
3.2.1.154 Fe3+ 1 mM, 90% inhibition Streptococcus mutans
3.2.1.154 Hg2+ 1 mM, complete inhibition Streptococcus mutans
3.2.1.154 Zn2+ 1 mM, 70% inhibition Streptococcus mutans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.154 extracellular
-
 Streptococcus mutans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 127000
-
 x * 127000, SDS-PAGE Streptococcus mutans
3.2.1.154 127000
-
 x * 127000, SDS-PAGE Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Streptococcus mutans
-
 ingbritt
-
3.2.1.154 Streptococcus mutans
-
 ingbritt
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80
-
 Streptococcus mutans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.154 culture supernatant
-
 Streptococcus mutans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.154 13.9
-
-
 Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + H2O
-
 Streptococcus mutans D-fructose + ?
-
 ?
3.2.1.80 raffinose + H2O
-
 Streptococcus mutans D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
 ?
3.2.1.154 inulin + H2O 13% of the activity with levan Streptococcus mutans fructose + ?
-
 ?
3.2.1.154 levan + H2O exo-type mechanism Streptococcus mutans fructose + ?
-
 ?
3.2.1.154 raffinose + H2O 5% of the activity with levan Streptococcus mutans ?
-
 ?
3.2.1.154 sucrose + H2O 9% of the activity with levan Streptococcus mutans D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.80 ? x * 127000, SDS-PAGE Streptococcus mutans
3.2.1.154 ? x * 127000, SDS-PAGE Streptococcus mutans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 55
-
 levan Streptococcus mutans
3.2.1.154 55
-
 hydrolysis of levan Streptococcus mutans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.80 40 60
-
 Streptococcus mutans
3.2.1.154 55 60 55°C: maximal activity, 60°C: 61% of maximal activity Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 5
-
 sucrose Streptococcus mutans
3.2.1.80 5.5
-
 levan Streptococcus mutans
3.2.1.80 6
-
 linulin Streptococcus mutans
3.2.1.154 5
-
 hydrolysis of sucrose Streptococcus mutans
3.2.1.154 5.5
-
 hydrolysis of levan Streptococcus mutans
3.2.1.154 6
-
 hydrolysis of inulin Streptococcus mutans