EC Number | General Stability | Organism |
---|---|---|
5.1.1.15 | freezing inactivates, particularly in dilute solution of less than 1 mg/ml | Achromobacter obae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.15 | 2-Methyl-3-benzothiazolonehydrazone hydrochloride | - |
Achromobacter obae | |
5.1.1.15 | Ala | weak | Achromobacter obae | |
5.1.1.15 | CuSO4 | - |
Achromobacter obae | |
5.1.1.15 | D-cycloserine | - |
Achromobacter obae | |
5.1.1.15 | epsilon-Caprolactam | - |
Achromobacter obae | |
5.1.1.15 | Glu | weak | Achromobacter obae | |
5.1.1.15 | Gly | - |
Achromobacter obae | |
5.1.1.15 | HgCl2 | - |
Achromobacter obae | |
5.1.1.15 | hydroxylamine | - |
Achromobacter obae | |
5.1.1.15 | L-His | - |
Achromobacter obae | |
5.1.1.15 | L-Lys | - |
Achromobacter obae | |
5.1.1.15 | L-Met | - |
Achromobacter obae | |
5.1.1.15 | L-Orn | - |
Achromobacter obae | |
5.1.1.15 | Leu | weak | Achromobacter obae | |
5.1.1.15 | NEM | - |
Achromobacter obae | |
5.1.1.15 | PCMB | - |
Achromobacter obae | |
5.1.1.15 | phenylhydrazine | - |
Achromobacter obae | |
5.1.1.15 | Pro | weak | Achromobacter obae | |
5.1.1.15 | Sodium borohydride | - |
Achromobacter obae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.1.15 | 6 | - |
L-alpha-amino-epsilon-caprolactam | - |
Achromobacter obae | |
5.1.1.15 | 8 | - |
D-alpha-Amino-epsilon-caprolactam | - |
Achromobacter obae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.1.1.15 | cytoplasm | - |
Achromobacter obae | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.1.15 | 49000 | - |
1 * 49000, SDS-PAGE | Achromobacter obae |
5.1.1.15 | 51000 | - |
gel filtration | Achromobacter obae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.15 | Achromobacter obae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.1.15 | - |
Achromobacter obae |
EC Number | Storage Stability | Organism |
---|---|---|
5.1.1.15 | -20°C, 0.25 M sucrose, 10 mM potassium phosphate buffer, pH 7.3, 0.02 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, protein concentration: more than 0.3%, stable for at least 6 months | Achromobacter obae |
5.1.1.15 | 20°C, 0.25 M sucrose, enzyme retains approximately 90% of its initial activity after storage for 4 months | Achromobacter obae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.15 | L-alpha-Amino-epsilon-caprolactam | r | Achromobacter obae | D-alpha-Amino-epsilon-caprolactam | - |
? | |
5.1.1.15 | additional information | the enzyme catalyzes alpha-proton exchange of the substrate with deuterium during racemization in deuterium oxide | Achromobacter obae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.1.15 | monomer | 1 * 49000, SDS-PAGE | Achromobacter obae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.1.15 | 60 | - |
pH 7.2, 20 min, stable | Achromobacter obae |
5.1.1.15 | 65 | - |
pH 7.2, 20 min, 5% loss of activity | Achromobacter obae |
5.1.1.15 | 70 | - |
pH 7.2, 20 min, 15% loss of activity | Achromobacter obae |
5.1.1.15 | 75 | - |
pH 7.2, 20 min, 40% loss of activity | Achromobacter obae |
5.1.1.15 | 80 | - |
pH 7.2, 20 min, 25% loss of activity | Achromobacter obae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.1.15 | 8.8 | - |
L-alpha-amino-epsilon-caprolactam | Achromobacter obae |
5.1.1.15 | 8.8 | - |
D-alpha-amino-epsilon-caprolactam | Achromobacter obae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.1.1.15 | 6 | 9 | at 25°C, 20 min, stable | Achromobacter obae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.15 | pyridoxal 5'-phosphate | enzyme contains 1 mol of pyridoxal 5'-phosphate per mol of enzyme. Km: 0.00021 mM | Achromobacter obae |