BRENDA - Enzyme Database

Lactase-phlorizin hydrolase complex from monkey small intestine: Stimulation of phlorizin hydrolase activity by organic acids

Hamaswamy, S.; Radhakrishnan, A.N.; Biochem. Biophys. Res. Commun. 54, 197-204 (1973)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.62
cis-aconitic acid
more than 2fold stimulation only for phlorizin activity
Macaca radiata
3.2.1.62
Citric acid
highest stimulation at 50 mM and 30░C only for phlorizin activity
Macaca radiata
3.2.1.62
isocitric acid
1.7fold stimulation only for phlorizin activity
Macaca radiata
3.2.1.62
L-ascorbic acid
1.6fold stimulation only for phlorizin activity
Macaca radiata
3.2.1.62
L-Tartaric acid
most stimulating effect only for phlorizin activity
Macaca radiata
3.2.1.62
Pyruvic acid
0.5fold stimulation only for phlorizin activity
Macaca radiata
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.62
2-oxoglutaric acid
50% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
Maleic acid
55% inhibition for the tartaric acid-stimulated phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
NaCl
30% inhibition of the tartaric acid-stimulated phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
oxalacetic acid
50% inhibition of the tartaric acid-stimualted phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
oxalic acid
47% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
Pyruvic acid
strongly inhibitory for the tartaric acid-stimulated phlorizin hydrolase
Macaca radiata
3.2.1.62
SO42-
strong inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
Macaca radiata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.62
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
Macaca radiata
-
D-galactose + beta-D-glucose
-
Macaca radiata
?
3.2.1.62
phlorizin + H2O
Macaca radiata
-
phloretin + beta-D-glucose
-
Macaca radiata
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.62
Macaca radiata
-
monkey
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.62
highly purified
Macaca radiata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.62
intestine
small intestine
Macaca radiata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.62
5
6
lactase activity
Macaca radiata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.62
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
-
208763
Macaca radiata
D-galactose + beta-D-glucose
-
208763
Macaca radiata
?
3.2.1.62
phlorizin + H2O
-
208763
Macaca radiata
phloretin + beta-D-glucose
-
208763
Macaca radiata
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.62
5.2
5.6
phlorizin activity in acetate buffer
Macaca radiata
3.2.1.62
5.6
-
phlorizin activity at pH 3.3-3.5 in citrate buffer
Macaca radiata
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.62
cis-aconitic acid
more than 2fold stimulation only for phlorizin activity
Macaca radiata
3.2.1.62
Citric acid
highest stimulation at 50 mM and 30░C only for phlorizin activity
Macaca radiata
3.2.1.62
isocitric acid
1.7fold stimulation only for phlorizin activity
Macaca radiata
3.2.1.62
L-ascorbic acid
1.6fold stimulation only for phlorizin activity
Macaca radiata
3.2.1.62
L-Tartaric acid
most stimulating effect only for phlorizin activity
Macaca radiata
3.2.1.62
Pyruvic acid
0.5fold stimulation only for phlorizin activity
Macaca radiata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.62
2-oxoglutaric acid
50% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
Maleic acid
55% inhibition for the tartaric acid-stimulated phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
NaCl
30% inhibition of the tartaric acid-stimulated phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
oxalacetic acid
50% inhibition of the tartaric acid-stimualted phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
oxalic acid
47% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
Macaca radiata
3.2.1.62
Pyruvic acid
strongly inhibitory for the tartaric acid-stimulated phlorizin hydrolase
Macaca radiata
3.2.1.62
SO42-
strong inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
Macaca radiata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.62
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
Macaca radiata
-
D-galactose + beta-D-glucose
-
Macaca radiata
?
3.2.1.62
phlorizin + H2O
Macaca radiata
-
phloretin + beta-D-glucose
-
Macaca radiata
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.62
highly purified
Macaca radiata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.62
intestine
small intestine
Macaca radiata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.62
5
6
lactase activity
Macaca radiata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.62
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
-
208763
Macaca radiata
D-galactose + beta-D-glucose
-
208763
Macaca radiata
?
3.2.1.62
phlorizin + H2O
-
208763
Macaca radiata
phloretin + beta-D-glucose
-
208763
Macaca radiata
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.62
5.2
5.6
phlorizin activity in acetate buffer
Macaca radiata
3.2.1.62
5.6
-
phlorizin activity at pH 3.3-3.5 in citrate buffer
Macaca radiata