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Literature summary extracted from

  • Leese, H.J.; Semenza, G.
    On the identity between the small intestine enzymes phlorizin hydrolase and glycosylceramidase (1973), J. Biol. Chem., 248, 8170-8173.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.62 cellobiose inhibition of galactosylceramide hydrolysis Rattus sp.
3.2.1.62 galactosylceramide competitive inhibition of glucosyldihydroceramide hydrolysis Rattus sp.
3.2.1.62 glucosyldihydroceramide competitive inhibition of galactosylceramide hydrolysis Rattus sp.
3.2.1.62 phlorizin competitive inhibition of galactosylceramide hydrolysis, Ki: 0.35 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.62 0.51
-
 galactosylceramide
-
 Rattus sp.
3.2.1.62 0.62
-
 glucosyldihydroceramide
-
 Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Rattus sp. best substrate D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Rattus sp. two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O Rattus sp. cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.62 phlorizin + H2O Rattus sp.
-
 phloretin + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.62 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.62
-
 Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.62 intestine
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.62 20
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.62 4'-deoxyphlorizin + H2O low activity Rattus sp. 4'-deoxyphloritin + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O best substrate Rattus sp. D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis Rattus sp. D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis Rattus sp. D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Rattus sp. beta-D-glucose + D-glucose
-
 ?
3.2.1.62 galactosyl-N-palmitoylceramide + H2O
-
 Rattus sp. beta-D-galactose + N-palmitoylceramide
-
 ?
3.2.1.62 galactosyl-N-palmitoyldihydroceramide + H2O
-
 Rattus sp. beta-D-galactose + N-palmitoyldihydroceramide
-
 ?
3.2.1.62 galactosylceramide + H2O
-
 Rattus sp. beta-D-galactose + ceramide
-
 ?
3.2.1.62 glucosyl-N-lignoceroyldihydroceramide + H2O
-
 Rattus sp. beta-D-glucose + N-lignoceroyldihydroceramide
-
 ?
3.2.1.62 glucosyl-N-palmitoylceramide + H2O
-
 Rattus sp. beta-D-glucose + N-palmitoylceramide
-
 ?
3.2.1.62 glucosyl-N-palmitoyldihydroceramide + H2O
-
 Rattus sp. beta-D-glucose + N-palmitoyldihydroceramide
-
 ?
3.2.1.62 glucosyl-N-stearoyldihydroceramide + H2O
-
 Rattus sp. beta-D-glucose + N-stearoyldihydroceramide
-
 ?
3.2.1.62 glucosyldihydroceramide
-
 Rattus sp. beta-D-glucose + dihydroceramide
-
 ?
3.2.1.62 lactosyl-N-lignoceroyldihydroceramide + H2O
-
 Rattus sp. beta-D-glucose + D-galactose + N-lignoceroyldihydroceramide
-
 ?
3.2.1.62 lactosyl-N-palmitoyldihydroceramide + H2O
-
 Rattus sp. beta-D-glucose + D-galactose + N-palmitoyldihydroceramide
-
 ?
3.2.1.62 lactosyl-N-stearoyldihydroceramide + H2O
-
 Rattus sp. beta-glucose + D-galactose + N-stearoyldihydroceramide
-
 ?
3.2.1.62 phlorizin + H2O
-
 Rattus sp. phloretin + beta-D-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.62 46
-
 34-70% remaining activity after 30 min, depending on substrates Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.62 6
-
-
 Rattus sp.