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Literature summary extracted from
- Lactase-phlorizin hydrolase complex from monkey small intestine. Purification, properties and evidence for two catalytic sites (1975), Biochim. Biophys. Acta, 403, 446-455.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.62 | cerebroside | from brain, competitive inhibition of lactase activity, Ki: 1.11 mM | Macaca radiata |  |
| 3.2.1.62 | phlorizin | competitive inhibition of lactase activity, Ki: 0.18 mM | Macaca radiata | |
| 3.2.1.62 | sodium cholate | competitive inhibition of lactase activity, in the presence of bovine serum albumin | Macaca radiata | |
| 3.2.1.108 | cerebroside | bovine brain cerebroside | Platyrrhini | |
| 3.2.1.108 | phlorizin | competitive inhibitor of lactose hydrolysis | Platyrrhini | |
| 3.7.1.4 | brain cerebroside | competitive inhibition | Platyrrhini | |
| 3.7.1.4 | phlorizin | competitive inhibition | Platyrrhini | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.62 | 0.4 | - |
phlorizin | - |
Macaca radiata | |
| 3.2.1.62 | 21.8 | - |
4-O-beta-D-galactopyranosyl-D-glucopyranoside | - |
Macaca radiata | |
| 3.2.1.108 | 0.4 | - |
phlorizin | - |
Platyrrhini | |
| 3.2.1.108 | 2.78 | - |
cellobiose | - |
Platyrrhini | |
| 3.2.1.108 | 21.8 | - |
lactose | - |
Platyrrhini | |
| 3.7.1.4 | 0.4 | - |
phlorizin | - |
Platyrrhini | |
| 3.7.1.4 | 2.78 | - |
cellobiose | - |
Platyrrhini | |
| 3.7.1.4 | 21.8 | - |
lactose | - |
Platyrrhini | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | Macaca radiata | low activity | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | Macaca radiata | salicin | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Macaca radiata | best substrate | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Macaca radiata | two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Macaca radiata | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | phlorizin + H2O | Macaca radiata | low activity | phloretin + beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | Macaca radiata | - |
monkey | - |
| 3.2.1.108 | Platyrrhini | - |
lactase-phlorizin hydrolase complex | - |
| 3.7.1.4 | Platyrrhini | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.62 | to homogeneity, chromatography techniques | Macaca radiata |
| 3.2.1.108 | - |
Platyrrhini |
| 3.7.1.4 | - |
Platyrrhini |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | intestine | small intestine | Macaca radiata | - |
| 3.2.1.108 | small intestine | - |
Platyrrhini | - |
| 3.7.1.4 | small intestine | - |
Platyrrhini | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 0.45 | - |
phlorizin activity | Macaca radiata |
| 3.2.1.62 | 16.1 | - |
lactase activity | Macaca radiata |
| 3.2.1.108 | 16.1 | - |
lactase | Platyrrhini |
| 3.2.1.108 | 450 | - |
phlorizin hydrolase | Platyrrhini |
| 3.7.1.4 | 0.45 | - |
- |
Platyrrhini |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | low activity | Macaca radiata | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Macaca radiata | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Macaca radiata | 2-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Macaca radiata | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.62 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Macaca radiata | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | best substrate | Macaca radiata | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis | Macaca radiata | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis | Macaca radiata | D-galactose + beta-D-glucose | - |
? | |
| 3.2.1.62 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Macaca radiata | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.62 | beta-methylglucoside + H2O | - |
Macaca radiata | methanol + beta-D-glucose | - |
? | |
| 3.2.1.62 | N-palmitoyldihydroglucosylcerebroside + H2O | 1.5% of the activity towards lactose | Macaca radiata | beta-D-glucose + N-palmitoyldihydrocerebroside | - |
? | |
| 3.2.1.62 | phlorizin + H2O | low activity | Macaca radiata | phloretin + beta-D-glucose | - |
? | |
| 3.2.1.108 | cellobiose + H2O | 16% of the activity with lactose | Platyrrhini | 2 beta-D-glucose | - |
? | |
| 3.2.1.108 | lactose + H2O | - |
Platyrrhini | D-glucose + D-galactose | most active substrate | ? | |
| 3.2.1.108 | methyl-beta-D-glucoside + H2O | 7.3% of the activity with lactose | Platyrrhini | methanol + beta-D-glucose | - |
? | |
| 3.2.1.108 | o-nitrophenyl-beta-galactoside + H2O | 15.4% of the activity with lactose | Platyrrhini | o-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.108 | o-nitrophenyl-beta-glucoside + H2O | 13% of the activity with lactose | Platyrrhini | o-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.108 | p-nitrophenyl-beta-galactoside + H2O | 9.3% of the activity with lactose | Platyrrhini | p-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.108 | p-nitrophenyl-beta-glucoside + H2O | 12.7% of the activity with lactose | Platyrrhini | p-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.108 | phlorizin + H2O | at 2.3% of the activity with lactose | Platyrrhini | phloretin + D-glucose | - |
? | |
| 3.2.1.108 | salicin + H2O | 1.6% of the activity with lactose | Platyrrhini | ? | - |
? | |
| 3.7.1.4 | beta-methyl D-glucoside + H2O | - |
Platyrrhini | ? | - |
? | |
| 3.7.1.4 | cellobiose + H2O | - |
Platyrrhini | 2 D-glucose | - |
? | |
| 3.7.1.4 | cerebrosides + H2O | - |
Platyrrhini | ? | - |
? | |
| 3.7.1.4 | lactose + H2O | - |
Platyrrhini | D-galactose + D-glucose | - |
? | |
| 3.7.1.4 | N-palmitoyldihydroglucosylceramide + H2O | - |
Platyrrhini | ? | - |
? | |
| 3.7.1.4 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Platyrrhini | o-nitrophenol + beta-D-galactose | - |
? | |
| 3.7.1.4 | o-nitrophenyl-beta-D-glucoside + H2O | - |
Platyrrhini | o-nitrophenol + beta-D-glucose | - |
? | |
| 3.7.1.4 | p-nitrophenyl-beta-D-galactoside + H2O | - |
Platyrrhini | p-nitrophenol + beta-D-galactose | - |
? | |
| 3.7.1.4 | p-nitrophenyl-beta-D-glucoside + H2O | - |
Platyrrhini | p-nitrophenol + beta-D-glucose | - |
? | |
| 3.7.1.4 | phloretin + H2O | - |
Platyrrhini | phloretate + phloroglucinol | - |
? | |
| 3.7.1.4 | phlorhizin + H2O | - |
Platyrrhini | 3-(4-hydroxyphenyl)propanoate + arbutin | - |
? | |
| 3.7.1.4 | salicin + H2O | - |
Platyrrhini | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 55 | - |
complete inactivation | Macaca radiata |
| 3.2.1.108 | 55 | - |
lactase and phlorizin hydrolase activity are identically heat inactivated | Platyrrhini |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 5.6 | - |
lactase activity, phlorizin activity in the acidic pH range | Macaca radiata |
| 3.7.1.4 | 5.6 | - |
- |
Platyrrhini |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.62 | 1.11 | - |
cerebroside | from brain, competitive inhibition of lactase activity | Macaca radiata | |
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