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Literature summary extracted from

  • Skovbjerg, H.; Sjöström, H.; Noren, O.
    Purification and characterization of amphiphilic lactase/phlorizin hydrolase from human small intestine (1981), Eur. J. Biochem., 114, 653-661.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.62 sensitive to repeated freezing and thawing Homo sapiens
3.2.1.108 repeated freezing and thawing, activity declines fast Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.62 phlorizin competitive inhibition of lactase activity; Ki: 0.44 mM Homo sapiens
3.2.1.62 Tris(hydroxymethyl)aminomethane competitive inhibition of lactase acitiviy, phlorizin activity not affected, Ki: 12 mM Homo sapiens
3.2.1.108 phlorizin
-
 Homo sapiens
3.2.1.108 Tris competitive Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.62 0.44
-
 phlorizin
-
 Homo sapiens
3.2.1.62 4.4
-
 4-O-beta-D-glucopyranosyl-D-glucospyranoside
-
 Homo sapiens
3.2.1.62 21
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
 Homo sapiens
3.2.1.108 0.44
-
 phlorizin
-
 Homo sapiens
3.2.1.108 4.4
-
 cellobiose
-
 Homo sapiens
3.2.1.108 21
-
 lactose
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.62 microvillus
-
 Homo sapiens 5902
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.62 160000
-
 2 * 160000, SDS-PAGE Homo sapiens
3.2.1.62 280000
-
 gel filtration, hydrophilic form Homo sapiens
3.2.1.62 320000
-
 gel filtration, amphiphilic form Homo sapiens
3.2.1.108 160000
-
 2 * 160000, SDS-PAGE Homo sapiens
3.2.1.108 280000
-
 hydrophilic enzyme form, gel filtration Homo sapiens
3.2.1.108 320000
-
 amphiphilic enzyme form, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Homo sapiens best substrate D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens cellobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.62 6-O-beta-D-glucopyranosyl-D-glucose + H2O Homo sapiens gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.62 phlorizin + H2O Homo sapiens
-
 phloretin + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.62 Homo sapiens
-
-
-
3.2.1.108 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.62 to homogeneity, immunoadsorbent chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.62 intestine small intestine Homo sapiens
-
3.2.1.108 small intestine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.62 32
-
-
 Homo sapiens
3.2.1.108 32
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.62 -20°C, several months Homo sapiens
3.2.1.108 -20°C, stable for several months Homo sapiens
3.2.1.108 4°C, stable for several weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.62 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Homo sapiens 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.62 4-nitrophenyl beta-D-galactopyranoside + H2O low activity Homo sapiens 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.62 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O best substrate Homo sapiens D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Homo sapiens beta-D-glucose + D-glucose
-
 ?
3.2.1.62 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Homo sapiens beta-D-glucose + D-glucose
-
 ?
3.2.1.62 phlorizin + H2O
-
 Homo sapiens phloretin + beta-D-glucose
-
 ?
3.2.1.108 cellobiose + H2O 14% of the activity with lactose Homo sapiens 2 beta-D-glucose
-
 ?
3.2.1.108 cellulose + H2O
-
 Homo sapiens beta-D-glucose
-
 ?
3.2.1.108 gentiobiose + H2O 0.7% of the activity with lactose Homo sapiens 2 beta-D-glucose
-
 ?
3.2.1.108 lactose + H2O
-
 Homo sapiens D-glucose + D-galactose
-
 ?
3.2.1.108 o-nitrophenyl-beta-galactoside + H2O 17% of the activity with lactose Homo sapiens o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.108 p-nitrophenyl-beta-galactoside + H2O 13% of the activity with lactose Homo sapiens p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.108 p-nitrophenyl-beta-glucoside + H2O 10% of the activity with lactose Homo sapiens p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.108 phenyl-beta-galactoside + H2O 0.1% of the activity with lactose Homo sapiens phenol + beta-D-galactose
-
 ?
3.2.1.108 phlorizin + H2O 22% of the activity with lactose Homo sapiens phloretin + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.62 dimer 2 * 160000, SDS-PAGE Homo sapiens
3.2.1.108 dimer 2 * 160000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.62 4
-
 no detectable loss of activity for several weeks Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.62 5.8 6
-
 Homo sapiens
3.2.1.108 5.8 6 hydrolysis of lactose Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.108 5 7 pH 5.0: about 70% of maximal activity, pH 7.0: about 65% of maximal activity Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.62 4.2 9 little activity outside this range Homo sapiens