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Literature summary extracted from
- Impact of O-glycosylation on the function of human intestinal lactase-phlorizin hydrolase. Characterization of glycoforms varying in enzyme activity and localization of O-glycoside addition (1992), J. Biol. Chem., 267, 25494-25504.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.62 | 14 | - |
4-O-beta-D-galactopyranosyl-D-glucopyranoside | both mannose-rich precursor form and mature form | Homo sapiens |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.62 | 160000 | - |
cleaved mature form, SDS-PAGE | Homo sapiens |
| 3.2.1.62 | 215000 | - |
mannose-rich precursor form, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Homo sapiens | 4fold higher activity for the N- and O-glycosylated than for the N-glycosylated molecule | D-galactose + beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.62 | glycoprotein | mature LPH O- and N-glycosylated, no sialic acids | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.62 | Helix pomatia lectin chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | intestine | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | 4fold higher activity for the N- and O-glycosylated than for the N-glycosylated molecule | Homo sapiens | D-galactose + beta-D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.62 | LPH | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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