BRENDA - Enzyme Database

Purification and properties of an alpha-L-fucosidase from rat epididymis

Carlsen, R.B.; Pierce, J.G.; J. Biol. Chem. 247, 23-32 (1972)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
Ag+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Cd2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Cu2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Hg2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Ni2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Pb2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Zn-acetate
more than 90% inhibition at 5 mM
Rattus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
47000
-
2 * 60000 + 2 * 2 * 47000, SDS-PAGE
Rattus sp.
3.2.1.51
60000
-
2 * 60000 + 2 * 2 * 47000, SDS-PAGE
Rattus sp.
3.2.1.51
210000
220000
sedimentation equilibrium centrifugation
Rattus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
IgG Fc peptide + H2O
Rattus sp.
human, rabbit, horse
alpha-L-fucose + ?
-
Rattus sp.
?
3.2.1.51
IgG L peptide + H2O
Rattus sp.
-
alpha-L-fucose + ?
-
Rattus sp.
?
3.2.1.51
LH-beta peptic glycopeptide + H2O
Rattus sp.
-
alpha-L-fucose + ?
-
Rattus sp.
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Rattus sp.
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
0.6% carbohydrate per native molecule
Rattus sp.
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
near homogeneity
Rattus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
epididymis
-
Rattus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
12.8
-
-
Rattus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208740
Rattus sp.
4-nitrophenol + alpha-L-fucose
-
208740
Rattus sp.
?
3.2.1.51
IgG Fc peptide + H2O
from human, rabbit, horse
208740
Rattus sp.
alpha-L-fucose + ?
-
208740
Rattus sp.
?
3.2.1.51
IgG Fc peptide + H2O
human, rabbit, horse
208740
Rattus sp.
alpha-L-fucose + ?
-
208740
Rattus sp.
?
3.2.1.51
IgG L peptide + H2O
-
208740
Rattus sp.
alpha-L-fucose + ?
-
208740
Rattus sp.
?
3.2.1.51
LH-beta peptic glycopeptide + H2O
-
208740
Rattus sp.
alpha-L-fucose + ?
-
208740
Rattus sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
2 * 60000 + 2 * 2 * 47000, SDS-PAGE
Rattus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
6.3
-
-
Rattus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
Ag+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Cd2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Cu2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Hg2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Ni2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Pb2+
effective inhibition at 5 mM
Rattus sp.
3.2.1.51
Zn-acetate
more than 90% inhibition at 5 mM
Rattus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
47000
-
2 * 60000 + 2 * 2 * 47000, SDS-PAGE
Rattus sp.
3.2.1.51
60000
-
2 * 60000 + 2 * 2 * 47000, SDS-PAGE
Rattus sp.
3.2.1.51
210000
220000
sedimentation equilibrium centrifugation
Rattus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
IgG Fc peptide + H2O
Rattus sp.
human, rabbit, horse
alpha-L-fucose + ?
-
Rattus sp.
?
3.2.1.51
IgG L peptide + H2O
Rattus sp.
-
alpha-L-fucose + ?
-
Rattus sp.
?
3.2.1.51
LH-beta peptic glycopeptide + H2O
Rattus sp.
-
alpha-L-fucose + ?
-
Rattus sp.
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
0.6% carbohydrate per native molecule
Rattus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
near homogeneity
Rattus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
epididymis
-
Rattus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
12.8
-
-
Rattus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208740
Rattus sp.
4-nitrophenol + alpha-L-fucose
-
208740
Rattus sp.
?
3.2.1.51
IgG Fc peptide + H2O
from human, rabbit, horse
208740
Rattus sp.
alpha-L-fucose + ?
-
208740
Rattus sp.
?
3.2.1.51
IgG Fc peptide + H2O
human, rabbit, horse
208740
Rattus sp.
alpha-L-fucose + ?
-
208740
Rattus sp.
?
3.2.1.51
IgG L peptide + H2O
-
208740
Rattus sp.
alpha-L-fucose + ?
-
208740
Rattus sp.
?
3.2.1.51
LH-beta peptic glycopeptide + H2O
-
208740
Rattus sp.
alpha-L-fucose + ?
-
208740
Rattus sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
2 * 60000 + 2 * 2 * 47000, SDS-PAGE
Rattus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
6.3
-
-
Rattus sp.