BRENDA - Enzyme Database

Studies on human polymorphonuclear leukocyte enzymes. IV. Intracellular distribution and properties of alpha-L-fucosidase

Avila, J.L.; Convit, J.; Biochim. Biophys. Acta 358, 308-318 (1974)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.51
KCl
17% activation at 300 mM
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
4-chloromercuribenzoate
92% inhibition at 0.1 mM
Homo sapiens
3.2.1.51
Ag+
59% inhibition at 3 mM
Homo sapiens
3.2.1.51
Cu2+
88% inhibition at 2 mM
Homo sapiens
3.2.1.51
D-glucose
21% inhibition at 6 mM
Homo sapiens
3.2.1.51
deoxycholate
36% inhibition at 0.012%, w/v
Homo sapiens
3.2.1.51
Fe3+
40% inhibition at 2 mM
Homo sapiens
3.2.1.51
formaldehyde
57% inhibition at 160 mM
Homo sapiens
3.2.1.51
Hg2+
92% inhibition at 0.1 mM
Homo sapiens
3.2.1.51
L-cysteine
20% inhibition at 30 mM
Homo sapiens
3.2.1.51
Mn2+
26% inhibition at 2 mM
Homo sapiens
3.2.1.51
Sn2+
24% inhibition at 2 mM
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.168
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
lysosome
-
Homo sapiens
5764
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.51
Fe2+
12% activation at 2 mM
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
leukocyte
-
Homo sapiens
-
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
-10C, 3-6% loss of activity after 3 days
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208737
Homo sapiens
4-nitrophenol + alpha-L-fucose
-
208737
Homo sapiens
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.2.1.51
45
-
-
Homo sapiens
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.2.1.51
4
55
highly stable
Homo sapiens
3.2.1.51
70
-
60% loss of activity
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.4
5.6
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.51
KCl
17% activation at 300 mM
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
4-chloromercuribenzoate
92% inhibition at 0.1 mM
Homo sapiens
3.2.1.51
Ag+
59% inhibition at 3 mM
Homo sapiens
3.2.1.51
Cu2+
88% inhibition at 2 mM
Homo sapiens
3.2.1.51
D-glucose
21% inhibition at 6 mM
Homo sapiens
3.2.1.51
deoxycholate
36% inhibition at 0.012%, w/v
Homo sapiens
3.2.1.51
Fe3+
40% inhibition at 2 mM
Homo sapiens
3.2.1.51
formaldehyde
57% inhibition at 160 mM
Homo sapiens
3.2.1.51
Hg2+
92% inhibition at 0.1 mM
Homo sapiens
3.2.1.51
L-cysteine
20% inhibition at 30 mM
Homo sapiens
3.2.1.51
Mn2+
26% inhibition at 2 mM
Homo sapiens
3.2.1.51
Sn2+
24% inhibition at 2 mM
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.168
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
lysosome
-
Homo sapiens
5764
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.51
Fe2+
12% activation at 2 mM
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
leukocyte
-
Homo sapiens
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
-10C, 3-6% loss of activity after 3 days
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208737
Homo sapiens
4-nitrophenol + alpha-L-fucose
-
208737
Homo sapiens
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.2.1.51
45
-
-
Homo sapiens
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.2.1.51
4
55
highly stable
Homo sapiens
3.2.1.51
70
-
60% loss of activity
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.4
5.6
-
Homo sapiens