BRENDA - Enzyme Database

Human liver alpha-L-fucosidase. Purification, characterization, and immunochemical studies

Alhadeff, J.A.; Miller, A.L.; Wenaas, H.; Vedvick, T.; O'Brien, J.S.; J. Biol. Chem. 250, 7106-7113 (1975)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.51
dithiothreitol
13% activation at 0.2 mM
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
Ag+
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
Homo sapiens
3.2.1.51
Hg2+
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.22
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
3.2.1.51
0.43
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.51
Mn2+
22% stimulation at 5 mM
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
230000
-
sedimentation equilibrium centrifugation
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
low activity
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
2'-fucosyllactose
alpha-L-fucose + lactose
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
1% carbohydrate content per molecule
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, 2step affinity chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
24.2
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
-20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208736
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208736
Homo sapiens
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208736
Homo sapiens
4-nitrophenol + alpha-L-fucose
-
208736
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
low activity
208736
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208736
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208736
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208736
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208736
Homo sapiens
alpha-L-fucose + lactose
-
208736
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
oligomer
subunits of 50.1 kDa each, SDS-PAGE
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
37
-
gradual loss of activity after 6 min for the purified enzyme, in crude state: stable for 4 h
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
4.6
-
one of two pH-optima
Homo sapiens
3.2.1.51
6.5
-
second pH-optimum
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.51
dithiothreitol
13% activation at 0.2 mM
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
Ag+
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
Homo sapiens
3.2.1.51
Hg2+
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.22
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
3.2.1.51
0.43
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.51
Mn2+
22% stimulation at 5 mM
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
230000
-
sedimentation equilibrium centrifugation
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
low activity
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
2'-fucosyllactose
alpha-L-fucose + lactose
-
Homo sapiens
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
1% carbohydrate content per molecule
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, 2step affinity chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
24.2
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
-20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208736
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208736
Homo sapiens
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208736
Homo sapiens
4-nitrophenol + alpha-L-fucose
-
208736
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
low activity
208736
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208736
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208736
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208736
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208736
Homo sapiens
alpha-L-fucose + lactose
-
208736
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
oligomer
subunits of 50.1 kDa each, SDS-PAGE
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
37
-
gradual loss of activity after 6 min for the purified enzyme, in crude state: stable for 4 h
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
4.6
-
one of two pH-optima
Homo sapiens
3.2.1.51
6.5
-
second pH-optimum
Homo sapiens