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Literature summary extracted from

  • Alhadeff, J.A.; Miller, A.L.; Wenaas, H.; Vedvick, T.; O'Brien, J.S.
    Human liver alpha-L-fucosidase. Purification, characterization, and immunochemical studies (1975), J. Biol. Chem., 250, 7106-7113.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.51 dithiothreitol 13% activation at 0.2 mM Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 Ag+ complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT Homo sapiens
3.2.1.51 Hg2+ complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.22
-
 4-methylumbelliferyl-alpha-L-fucopyranoside
-
 Homo sapiens
3.2.1.51 0.43
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.51 Mn2+ 22% stimulation at 5 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 230000
-
 sedimentation equilibrium centrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Homo sapiens low activity alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Homo sapiens lacto-N-fucopentaose I alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Homo sapiens 2'-fucosyllactose alpha-L-fucose + lactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein 1% carbohydrate content per molecule Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, 2step affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 24.2
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 -20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-L-fucose
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O low activity Homo sapiens alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaose I Homo sapiens alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O 2'-fucosyllactose Homo sapiens alpha-L-fucose + lactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 oligomer subunits of 50.1 kDa each, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 37
-
 gradual loss of activity after 6 min for the purified enzyme, in crude state: stable for 4 h Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 4.6
-
 one of two pH-optima Homo sapiens
3.2.1.51 6.5
-
 second pH-optimum Homo sapiens