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Literature summary extracted from

  • Di Matteo, G.; Orfeo, M.A.; Romeo, G.
    Human alpha-fucosidase. Purification and properties (1976), Biochim. Biophys. Acta, 429, 527-537.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.51 bovine serum albumin increases acitivity up to 100% Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.3
-
 4-methylumbelliferyl-alpha-L-fucopyranoside
-
 Homo sapiens
3.2.1.51 0.38
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 50000
-
 4 * 50000, gel filtration Homo sapiens
3.2.1.51 200000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, affinity chromatography, isoelectric focusing, 2 forms, alpha-L-fucosidase A and B Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 brain
-
 Homo sapiens
-
3.2.1.51 heart
-
 Homo sapiens
-
3.2.1.51 kidney
-
 Homo sapiens
-
3.2.1.51 placenta
-
 Homo sapiens
-
3.2.1.51 serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 1.8
-
 alpha-L-fucosidase B Homo sapiens
3.2.1.51 3.81
-
 alpha-L-fucosidase A Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-L-fucose
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + alpha-L-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 tetramer 4 * 50000, gel filtration Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 51
-
 rapid inactivation for alpha-L-fucosidase A, form B more heat-stable, stabilizing effect of bovine serum albumin, addition of 0.5 M potassium thiocyanate leads to more heat sensitivity for both forms Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5.5
-
 for 4-nitrophenyl-alpha-L-fucopyranoside Homo sapiens
3.2.1.51 6
-
 for 4-methylumbelliferyl-alpha-L-fucopyranoside Homo sapiens