BRENDA - Enzyme Database

Human alpha-fucosidase. Purification and properties

Di Matteo, G.; Orfeo, M.A.; Romeo, G.; Biochim. Biophys. Acta 429, 527-537 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.51
bovine serum albumin
increases acitivity up to 100%
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.3
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
3.2.1.51
0.38
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
50000
-
4 * 50000, gel filtration
Homo sapiens
3.2.1.51
200000
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
-
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography, isoelectric focusing, 2 forms, alpha-L-fucosidase A and B
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
brain
-
Homo sapiens
-
3.2.1.51
heart
-
Homo sapiens
-
3.2.1.51
kidney
-
Homo sapiens
-
3.2.1.51
placenta
-
Homo sapiens
-
3.2.1.51
serum
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
1.8
-
alpha-L-fucosidase B
Homo sapiens
3.2.1.51
3.81
-
alpha-L-fucosidase A
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208735
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208735
Homo sapiens
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208735
Homo sapiens
4-nitrophenol + alpha-L-fucose
-
208735
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 50000, gel filtration
Homo sapiens
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.2.1.51
51
-
rapid inactivation for alpha-L-fucosidase A, form B more heat-stable, stabilizing effect of bovine serum albumin, addition of 0.5 M potassium thiocyanate leads to more heat sensitivity for both forms
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
-
for 4-nitrophenyl-alpha-L-fucopyranoside
Homo sapiens
3.2.1.51
6
-
for 4-methylumbelliferyl-alpha-L-fucopyranoside
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.51
bovine serum albumin
increases acitivity up to 100%
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.3
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
3.2.1.51
0.38
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
50000
-
4 * 50000, gel filtration
Homo sapiens
3.2.1.51
200000
-
gel filtration
Homo sapiens
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
-
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography, isoelectric focusing, 2 forms, alpha-L-fucosidase A and B
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
brain
-
Homo sapiens
-
3.2.1.51
heart
-
Homo sapiens
-
3.2.1.51
kidney
-
Homo sapiens
-
3.2.1.51
placenta
-
Homo sapiens
-
3.2.1.51
serum
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
1.8
-
alpha-L-fucosidase B
Homo sapiens
3.2.1.51
3.81
-
alpha-L-fucosidase A
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208735
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208735
Homo sapiens
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208735
Homo sapiens
4-nitrophenol + alpha-L-fucose
-
208735
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 50000, gel filtration
Homo sapiens
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.2.1.51
51
-
rapid inactivation for alpha-L-fucosidase A, form B more heat-stable, stabilizing effect of bovine serum albumin, addition of 0.5 M potassium thiocyanate leads to more heat sensitivity for both forms
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
-
for 4-nitrophenyl-alpha-L-fucopyranoside
Homo sapiens
3.2.1.51
6
-
for 4-methylumbelliferyl-alpha-L-fucopyranoside
Homo sapiens