BRENDA - Enzyme Database

Glycosidases of molluscs. Purification and properties of alpha-L-fucosidase from Chamelea gallina L.

Reglero, A.; Cabezas, J.A.; Eur. J. Biochem. 66, 379-387 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.51
CN-
30% activation at 10 mM
Chamelea gallina
3.2.1.51
ethylenediaminetetraacetic acid
5% activation at 10 mM
Chamelea gallina
General Stability
EC Number
General Stability
Organism
3.2.1.51
sensitive to repeated freezing and thawing
Chamelea gallina
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competitive inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-fucose
15% inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-galactono-1,4-lactone
15% inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-galactose
15% inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-glucono-1,5-lactone
30% inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-glucose
15% inhibition at 50 mM
Chamelea gallina
3.2.1.51
Hg2+
effective non-competitive inhibition at 2 mM
Chamelea gallina
3.2.1.51
L-galactono-1,4-lactone
30% inhibition at 50 mM
Chamelea gallina
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.07
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Chamelea gallina
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.51
Ca2+
10% activation at 10 mM
Chamelea gallina
3.2.1.51
Zn2+
10% activation at 10 mM
Chamelea gallina
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
200000
-
gel filtration
Chamelea gallina
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal + H2O
Chamelea gallina
-
alpha-L-fucose + D-galactose
-
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Chamelea gallina
2'-fucosyllactose
alpha-L-fucose + lactose
-
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
Chamelea gallina
3-fucosyllactose
alpha-D-fucose + lactose
-
Chamelea gallina
?
3.2.1.51
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Chamelea gallina
lacto-N-fucopentaose III
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Chamelea gallina
?
3.2.1.51
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
Chamelea gallina
-
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
Chamelea gallina
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Chamelea gallina
-
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Chamelea gallina
?
3.2.1.51
oligosaccharide + H2O
Chamelea gallina
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
alpha-L-fucose + ?
-
-
?
3.2.1.51
submaxillary mucin + H2O
Chamelea gallina
-
alpha-L-fucose + ?
-
Chamelea gallina
?
3.2.1.51
thyroglobulin + H2O
Chamelea gallina
-
alpha-L-fucose + ?
-
Chamelea gallina
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Chamelea gallina
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, isoelectric focusing
Chamelea gallina
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
hepatopancreas
-
Chamelea gallina
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
1620
-
-
Chamelea gallina
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
208734
Chamelea gallina
4-nitrophenol + alpha-L-fucose
-
208734
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-2)-Gal + H2O
-
208734
Chamelea gallina
alpha-L-fucose + D-galactose
-
208734
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208734
Chamelea gallina
alpha-L-fucose + lactose
-
208734
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208734
Chamelea gallina
alpha-L-fucose + lactose
-
208734
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208734
Chamelea gallina
alpha-D-fucose + lactose
-
208734
Chamelea gallina
?
3.2.1.51
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose III
208734
Chamelea gallina
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208734
Chamelea gallina
?
3.2.1.51
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
-
208734
Chamelea gallina
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
208734
Chamelea gallina
?
3.2.1.51
lacto-N-fucopentaose II + H2O
-
208734
Chamelea gallina
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208734
Chamelea gallina
?
3.2.1.51
oligosaccharide + H2O
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
208734
Chamelea gallina
alpha-L-fucose + ?
-
-
-
?
3.2.1.51
oligosaccharides + H2O
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
208734
Chamelea gallina
alpha-L-fucose + ?
-
-
-
?
3.2.1.51
submaxillary mucin + H2O
-
208734
Chamelea gallina
alpha-L-fucose + ?
-
208734
Chamelea gallina
?
3.2.1.51
thyroglobulin + H2O
-
208734
Chamelea gallina
alpha-L-fucose + ?
-
208734
Chamelea gallina
?
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.2.1.51
65
-
fully active after 5 min
Chamelea gallina
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.2
-
-
Chamelea gallina
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
6
stable between
Chamelea gallina
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.51
CN-
30% activation at 10 mM
Chamelea gallina
3.2.1.51
ethylenediaminetetraacetic acid
5% activation at 10 mM
Chamelea gallina
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.1.51
sensitive to repeated freezing and thawing
Chamelea gallina
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competitive inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-fucose
15% inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-galactono-1,4-lactone
15% inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-galactose
15% inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-glucono-1,5-lactone
30% inhibition at 50 mM
Chamelea gallina
3.2.1.51
D-glucose
15% inhibition at 50 mM
Chamelea gallina
3.2.1.51
Hg2+
effective non-competitive inhibition at 2 mM
Chamelea gallina
3.2.1.51
L-galactono-1,4-lactone
30% inhibition at 50 mM
Chamelea gallina
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.07
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Chamelea gallina
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.51
Ca2+
10% activation at 10 mM
Chamelea gallina
3.2.1.51
Zn2+
10% activation at 10 mM
Chamelea gallina
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
200000
-
gel filtration
Chamelea gallina
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal + H2O
Chamelea gallina
-
alpha-L-fucose + D-galactose
-
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Chamelea gallina
2'-fucosyllactose
alpha-L-fucose + lactose
-
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
Chamelea gallina
3-fucosyllactose
alpha-D-fucose + lactose
-
Chamelea gallina
?
3.2.1.51
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Chamelea gallina
lacto-N-fucopentaose III
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Chamelea gallina
?
3.2.1.51
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
Chamelea gallina
-
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
Chamelea gallina
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Chamelea gallina
-
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Chamelea gallina
?
3.2.1.51
oligosaccharide + H2O
Chamelea gallina
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
alpha-L-fucose + ?
-
-
?
3.2.1.51
submaxillary mucin + H2O
Chamelea gallina
-
alpha-L-fucose + ?
-
Chamelea gallina
?
3.2.1.51
thyroglobulin + H2O
Chamelea gallina
-
alpha-L-fucose + ?
-
Chamelea gallina
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, isoelectric focusing
Chamelea gallina
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
hepatopancreas
-
Chamelea gallina
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
1620
-
-
Chamelea gallina
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
208734
Chamelea gallina
4-nitrophenol + alpha-L-fucose
-
208734
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-2)-Gal + H2O
-
208734
Chamelea gallina
alpha-L-fucose + D-galactose
-
208734
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208734
Chamelea gallina
alpha-L-fucose + lactose
-
208734
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208734
Chamelea gallina
alpha-L-fucose + lactose
-
208734
Chamelea gallina
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208734
Chamelea gallina
alpha-D-fucose + lactose
-
208734
Chamelea gallina
?
3.2.1.51
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose III
208734
Chamelea gallina
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208734
Chamelea gallina
?
3.2.1.51
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
-
208734
Chamelea gallina
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
208734
Chamelea gallina
?
3.2.1.51
lacto-N-fucopentaose II + H2O
-
208734
Chamelea gallina
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208734
Chamelea gallina
?
3.2.1.51
oligosaccharide + H2O
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
208734
Chamelea gallina
alpha-L-fucose + ?
-
-
-
?
3.2.1.51
oligosaccharides + H2O
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
208734
Chamelea gallina
alpha-L-fucose + ?
-
-
-
?
3.2.1.51
submaxillary mucin + H2O
-
208734
Chamelea gallina
alpha-L-fucose + ?
-
208734
Chamelea gallina
?
3.2.1.51
thyroglobulin + H2O
-
208734
Chamelea gallina
alpha-L-fucose + ?
-
208734
Chamelea gallina
?
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.2.1.51
65
-
fully active after 5 min
Chamelea gallina
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.2
-
-
Chamelea gallina
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
6
stable between
Chamelea gallina