BRENDA - Enzyme Database

The purification and characterization of rat liver lysosomal alpha-L-fucosidase

Opheim, D.J.; Touster, O.; J. Biol. Chem. 252, 739-743 (1977)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.1.51
stabilizing effect by addition of 0.05 mM/ml bovine serum albumin, 1 mM 2-mercaptoethanol, 1 mM MgSO4, high protein concentrations required
Rattus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 1.6 mM
Rattus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.19
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Rattus sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
lysosome
-
Rattus sp.
5764
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
55000
-
4 * 55000, SDS-PAGE
Rattus sp.
3.2.1.51
217000
-
sedimentation equilibrium centrifugation
Rattus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
glycopeptide + H2O
Rattus sp.
not active on native glycoproteins
alpha-L-fucose + ?
-
Rattus sp.
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Rattus sp.
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
residues per tetramer: fucose 3.5, mannose 32, galactose 8: glucose 9, glucosamine 32, sialic acid 8
Rattus sp.
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Rattus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
27
28
-
Rattus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
208733
Rattus sp.
4-nitrophenol + alpha-L-fucose
-
208733
Rattus sp.
?
3.2.1.51
glycopeptide + H2O
not active on native glycoproteins
208733
Rattus sp.
alpha-L-fucose + ?
-
208733
Rattus sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 55000, SDS-PAGE
Rattus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.7
5.9
-
Rattus sp.
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.1.51
stabilizing effect by addition of 0.05 mM/ml bovine serum albumin, 1 mM 2-mercaptoethanol, 1 mM MgSO4, high protein concentrations required
Rattus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 1.6 mM
Rattus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.19
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Rattus sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
lysosome
-
Rattus sp.
5764
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
55000
-
4 * 55000, SDS-PAGE
Rattus sp.
3.2.1.51
217000
-
sedimentation equilibrium centrifugation
Rattus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
glycopeptide + H2O
Rattus sp.
not active on native glycoproteins
alpha-L-fucose + ?
-
Rattus sp.
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
residues per tetramer: fucose 3.5, mannose 32, galactose 8: glucose 9, glucosamine 32, sialic acid 8
Rattus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Rattus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
27
28
-
Rattus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
208733
Rattus sp.
4-nitrophenol + alpha-L-fucose
-
208733
Rattus sp.
?
3.2.1.51
glycopeptide + H2O
not active on native glycoproteins
208733
Rattus sp.
alpha-L-fucose + ?
-
208733
Rattus sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 55000, SDS-PAGE
Rattus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.7
5.9
-
Rattus sp.