BRENDA - Enzyme Database

Purification of alpha-L-fucosidase from various sources by affinity chromatography

Jain, R.S.; Binder, R.L.; Levy-Benshimol, A.; Buck, C.A.; Warren, L.; J. Chromatogr. 139, 283-290 (1977)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
4-aminophenyl 1-thio alpha-L-fucoside
weak competitive inhibition, Ki: 1.25 mM
Rattus sp.
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 0.285 mM
Rattus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.277
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Rattus sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
-
Clostridium perfringens
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
gastric mucin + H2O
Clostridium perfringens
porcine
alpha-L-fucose + ?
-
Clostridium perfringens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Clostridium perfringens
-
-
-
3.2.1.51
Limulus polyphemus
-
-
-
3.2.1.51
Rattus sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Clostridium perfringens
3.2.1.51
to homogeneity, affinity chromatography
Limulus polyphemus
3.2.1.51
to homogeneity, affinity chromatography
Rattus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Clostridium perfringens
-
3.2.1.51
epididymis
-
Rattus sp.
-
3.2.1.51
serum
-
Limulus polyphemus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
0.39
-
-
Limulus polyphemus
3.2.1.51
2.61
-
-
Clostridium perfringens
3.2.1.51
15.6
-
-
Rattus sp.
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months
Limulus polyphemus
3.2.1.51
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months
Clostridium perfringens
3.2.1.51
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months
Rattus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208731
Limulus polyphemus
4-nitrophenol + alpha-L-fucose
-
208731
Limulus polyphemus
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208731
Rattus sp.
4-nitrophenol + alpha-L-fucose
-
208731
Rattus sp.
?
3.2.1.51
gastric mucin + H2O
porcine
208731
Clostridium perfringens
alpha-L-fucose + ?
-
208731
Clostridium perfringens
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
1.25
-
4-aminophenyl 1-thio alpha-L-fucoside
weak competitive inhibition
Rattus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
4-aminophenyl 1-thio alpha-L-fucoside
weak competitive inhibition, Ki: 1.25 mM
Rattus sp.
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 0.285 mM
Rattus sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
1.25
-
4-aminophenyl 1-thio alpha-L-fucoside
weak competitive inhibition
Rattus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.277
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Rattus sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
-
Clostridium perfringens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
gastric mucin + H2O
Clostridium perfringens
porcine
alpha-L-fucose + ?
-
Clostridium perfringens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Clostridium perfringens
3.2.1.51
to homogeneity, affinity chromatography
Limulus polyphemus
3.2.1.51
to homogeneity, affinity chromatography
Rattus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Clostridium perfringens
-
3.2.1.51
epididymis
-
Rattus sp.
-
3.2.1.51
serum
-
Limulus polyphemus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
0.39
-
-
Limulus polyphemus
3.2.1.51
2.61
-
-
Clostridium perfringens
3.2.1.51
15.6
-
-
Rattus sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months
Limulus polyphemus
3.2.1.51
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months
Clostridium perfringens
3.2.1.51
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months
Rattus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208731
Limulus polyphemus
4-nitrophenol + alpha-L-fucose
-
208731
Limulus polyphemus
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208731
Rattus sp.
4-nitrophenol + alpha-L-fucose
-
208731
Rattus sp.
?
3.2.1.51
gastric mucin + H2O
porcine
208731
Clostridium perfringens
alpha-L-fucose + ?
-
208731
Clostridium perfringens
?