BRENDA - Enzyme Database

alpha-L-Fucosidase from rat liver lysosomes

Opheim, D.J.; Touster, O.; Methods Enzymol. 50, 505-510 (1978)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.1.51
stabilizing effect by addition of 0.05 mM/ml bovine serum albumin, 1 mM 2-mercaptoethanol, 1 mM MgSO4, high protein concentrations required
Rattus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
4-chloromercuriphenylsulfonic acid
complete inhibition at 0.002 mM
Rattus sp.
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 1.6 mM
Rattus sp.
3.2.1.51
Cu2+
-
Rattus sp.
3.2.1.51
Hg2+
-
Rattus sp.
3.2.1.51
Ni2+
-
Rattus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.19
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Rattus sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
lysosome
-
Rattus sp.
5764
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
55000
-
4 * 55000, SDS-PAGE
Rattus sp.
3.2.1.51
217000
-
sedimentation equilibrium centrifugation
Rattus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Rattus sp.
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
residues per tetramer: fucose 3.5, mannose 32, galactose 8: glucose 9, glucosamine 32, sialic acid 8
Rattus sp.
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Rattus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
27
28
-
Rattus sp.
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
-20░C, acetate buffer, pH 6.0, 100 mM L-fucose, several months
Rattus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
208729
Rattus sp.
4-nitrophenol + alpha-L-fucose
-
208729
Rattus sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 55000, SDS-PAGE
Rattus sp.
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.2.1.51
25
-
50% remaining activity after 2 h
Rattus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
5.8
-
Rattus sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
8
stable in the presence of 0.05 mg/mg bovine serum albumin, 1 mM 2-mercaptoethanol
Rattus sp.
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.1.51
stabilizing effect by addition of 0.05 mM/ml bovine serum albumin, 1 mM 2-mercaptoethanol, 1 mM MgSO4, high protein concentrations required
Rattus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
4-chloromercuriphenylsulfonic acid
complete inhibition at 0.002 mM
Rattus sp.
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 1.6 mM
Rattus sp.
3.2.1.51
Cu2+
-
Rattus sp.
3.2.1.51
Hg2+
-
Rattus sp.
3.2.1.51
Ni2+
-
Rattus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.19
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Rattus sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
lysosome
-
Rattus sp.
5764
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
55000
-
4 * 55000, SDS-PAGE
Rattus sp.
3.2.1.51
217000
-
sedimentation equilibrium centrifugation
Rattus sp.
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
residues per tetramer: fucose 3.5, mannose 32, galactose 8: glucose 9, glucosamine 32, sialic acid 8
Rattus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Rattus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
liver
-
Rattus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
27
28
-
Rattus sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
-20░C, acetate buffer, pH 6.0, 100 mM L-fucose, several months
Rattus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
208729
Rattus sp.
4-nitrophenol + alpha-L-fucose
-
208729
Rattus sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 55000, SDS-PAGE
Rattus sp.
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
3.2.1.51
25
-
50% remaining activity after 2 h
Rattus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
5.8
-
Rattus sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
8
stable in the presence of 0.05 mg/mg bovine serum albumin, 1 mM 2-mercaptoethanol
Rattus sp.