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Literature summary extracted from

  • Cabezas, J.A.; Reglero, A.; Calvo, P.; Pedro de, M.A.
    Comparative stuy of alpha-L-fucosidase from three species os marine molluscs: Purification and properties (1979), Glycoconjugate Res., 2, 867-871.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 alpha-L-fucose competitive inhibition at 50 mM Chamelea gallina
3.2.1.51 alpha-L-fucose competitive inhibition at 50 mM Littorina littorea
3.2.1.51 alpha-L-fucose competitive inhibition at 50 mM Polititapes rhomboides
3.2.1.51 Hg2+ effective non-competitive inhibition at 2 mM Chamelea gallina
3.2.1.51 Hg2+ effective non-competitive inhibition at 2 mM Littorina littorea
3.2.1.51 Hg2+ effective non-competitive inhibition at 2 mM Polititapes rhomboides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.07
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Chamelea gallina
3.2.1.51 0.14
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Polititapes rhomboides
3.2.1.51 0.3
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Littorina littorea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 200000
-
 gel filtration Chamelea gallina
3.2.1.51 200000
-
 gel filtration Polititapes rhomboides
3.2.1.51 220000
-
 gel filtration Littorina littorea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 oligosaccharide + H2O Littorina littorea di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds alpha-L-fucose + ?
-
 ?
3.2.1.51 oligosaccharide + H2O Chamelea gallina di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds alpha-L-fucose + ?
-
 ?
3.2.1.51 submaxillary mucin + H2O Littorina littorea A-minus type and desialyzed, porcine alpha-L-fucose + ?
-
 ?
3.2.1.51 submaxillary mucin + H2O Chamelea gallina A-minus type and desialyzed, porcine alpha-L-fucose + ?
-
 ?
3.2.1.51 thyroglobulin + H2O Littorina littorea
-
 alpha-L-fucose + ?
-
 ?
3.2.1.51 thyroglobulin + H2O Chamelea gallina
-
 alpha-L-fucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Chamelea gallina
-
-
-
3.2.1.51 Littorina littorea
-
-
-
3.2.1.51 Polititapes rhomboides
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein contains 7.4% glucose, 2.2% galactose, 1.8% mannose, 2.2% hexosamine Littorina littorea

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, isoelectric focusing Littorina littorea
3.2.1.51 to homogeneity, isoelectric focusing Chamelea gallina
3.2.1.51 to homogeneity, isoelectric focusing Polititapes rhomboides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 hepatopancreas
-
 Littorina littorea
-
3.2.1.51 hepatopancreas
-
 Chamelea gallina
-
3.2.1.51 hepatopancreas
-
 Polititapes rhomboides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Littorina littorea 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Chamelea gallina 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Polititapes rhomboides 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 oligosaccharide + H2O di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds Littorina littorea alpha-L-fucose + ?
-
 ?
3.2.1.51 oligosaccharide + H2O di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds Chamelea gallina alpha-L-fucose + ?
-
 ?
3.2.1.51 oligosaccharides + H2O di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds Littorina littorea alpha-L-fucose + ?
-
 ?
3.2.1.51 oligosaccharides + H2O di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds Chamelea gallina alpha-L-fucose + ?
-
 ?
3.2.1.51 submaxillary mucin + H2O A-minus type and desialyzed, porcine Littorina littorea alpha-L-fucose + ?
-
 ?
3.2.1.51 submaxillary mucin + H2O A-minus type and desialyzed, porcine Chamelea gallina alpha-L-fucose + ?
-
 ?
3.2.1.51 thyroglobulin + H2O
-
 Littorina littorea alpha-L-fucose + ?
-
 ?
3.2.1.51 thyroglobulin + H2O
-
 Chamelea gallina alpha-L-fucose + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 60
-
 fully active for 5 min Littorina littorea
3.2.1.51 60
-
 fully active for 5 min Chamelea gallina
3.2.1.51 60
-
 fully active for 5 min Polititapes rhomboides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5.2
-
-
 Chamelea gallina
3.2.1.51 5.3
-
-
 Polititapes rhomboides
3.2.1.51 6.5
-
-
 Littorina littorea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 4 6 stable between Chamelea gallina
3.2.1.51 4 6 stable between Polititapes rhomboides
3.2.1.51 5 8 stable between Littorina littorea