BRENDA - Enzyme Database

Purification and characterization of I-cell disease alpha-L-fucosidase

Kress, B.C.; Freeze, H.H.; Herd, J.K.; Alhadeff, J.A.; Miller, A.L.; J. Biol. Chem. 255, 955-961 (1980)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.05
0.06
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
altered carbohydrate composition from enzymes of liver I-cell disease or pseudo-Hurler polydystrophy urine samples
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
liver
normal and I-cell disease samples
Homo sapiens
-
3.2.1.51
urine
samples from pseudo-Hurler polydystrophy
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
39.7
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208724
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208724
Homo sapiens
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
3.9
4
major pH-optimum
Homo sapiens
3.2.1.51
6.9
-
second minor pH-optimum
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.05
0.06
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
altered carbohydrate composition from enzymes of liver I-cell disease or pseudo-Hurler polydystrophy urine samples
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
liver
normal and I-cell disease samples
Homo sapiens
-
3.2.1.51
urine
samples from pseudo-Hurler polydystrophy
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
39.7
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208724
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208724
Homo sapiens
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
3.9
4
major pH-optimum
Homo sapiens
3.2.1.51
6.9
-
second minor pH-optimum
Homo sapiens