BRENDA - Enzyme Database

Purification and properties of two forms of human alpha-L-fucosidase

Chien, S.F.; Dawson, G.; Biochim. Biophys. Acta 614, 476-488 (1980)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.083
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
3.2.1.51
0.125
-
4-methylumbelliferyl-alpha-L-fucopyranoside
alpha-fucosidase I
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
50000
-
alpha-fucosidase II, gel filtration
Homo sapiens
3.2.1.51
100000
-
gel filtration, alpha-fucosidase I
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
2'-fucosyllactose
alpha-L-fucose + lactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Homo sapiens
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Homo sapiens
preferentially hydrolysed by alpha-fucosidase II
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
alpha-fucosidase I: sialoglycoprotein, mannose-rich: alpha-fucosidase II
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, two forms: alpha-fucosidase I and II, affinity chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
brain
-
Homo sapiens
-
3.2.1.51
kidney
-
Homo sapiens
-
3.2.1.51
liver
-
Homo sapiens
-
3.2.1.51
spleen
-
Homo sapiens
-
3.2.1.51
urine
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208721
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208721
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208721
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208721
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208721
Homo sapiens
alpha-L-fucose + lactose
-
208721
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
-
208721
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosamine
-
208721
Homo sapiens
?
3.2.1.51
lacto-N-fucopentaose II + H2O
preferentially hydrolysed by alpha-fucosidase II
208721
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208721
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
dimer
alpha-fucosidase I, 2 * 50000, SDS-PAGE, can aggregate to tetra- and hexamers
Homo sapiens
3.2.1.51
monomer
alpha-fucosidase II, 1 * 50000, SDS-PAGE, less-biologically active form, can aggregate to tetra- and hexamers
Homo sapiens
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.2.1.51
50
-
alpha-fucosidase I: 50% loss of activity in 40 min, alpha-fucosidase II: 20% loss of activity in 40 min
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
-
alpha-fucosidase II
Homo sapiens
3.2.1.51
5.3
-
alpha-fucosidase I
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.083
-
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
3.2.1.51
0.125
-
4-methylumbelliferyl-alpha-L-fucopyranoside
alpha-fucosidase I
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
50000
-
alpha-fucosidase II, gel filtration
Homo sapiens
3.2.1.51
100000
-
gel filtration, alpha-fucosidase I
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
2'-fucosyllactose
alpha-L-fucose + lactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Homo sapiens
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Homo sapiens
preferentially hydrolysed by alpha-fucosidase II
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Homo sapiens
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
alpha-fucosidase I: sialoglycoprotein, mannose-rich: alpha-fucosidase II
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, two forms: alpha-fucosidase I and II, affinity chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
brain
-
Homo sapiens
-
3.2.1.51
kidney
-
Homo sapiens
-
3.2.1.51
liver
-
Homo sapiens
-
3.2.1.51
spleen
-
Homo sapiens
-
3.2.1.51
urine
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208721
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208721
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208721
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208721
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208721
Homo sapiens
alpha-L-fucose + lactose
-
208721
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
-
208721
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosamine
-
208721
Homo sapiens
?
3.2.1.51
lacto-N-fucopentaose II + H2O
preferentially hydrolysed by alpha-fucosidase II
208721
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208721
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
dimer
alpha-fucosidase I, 2 * 50000, SDS-PAGE, can aggregate to tetra- and hexamers
Homo sapiens
3.2.1.51
monomer
alpha-fucosidase II, 1 * 50000, SDS-PAGE, less-biologically active form, can aggregate to tetra- and hexamers
Homo sapiens
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.2.1.51
50
-
alpha-fucosidase I: 50% loss of activity in 40 min, alpha-fucosidase II: 20% loss of activity in 40 min
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
-
alpha-fucosidase II
Homo sapiens
3.2.1.51
5.3
-
alpha-fucosidase I
Homo sapiens