BRENDA - Enzyme Database

Porcine thyroid fucosidase

Grove, D.S.; Serif, G.S.; Biochim. Biophys. Acta 662, 246-255 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate
complete inactivation at 22 mM
Sus scrofa
3.2.1.51
4-mercuribenzoate
complete inhibition at 0.25 mM
Sus scrofa
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 0.13 mM
Sus scrofa
3.2.1.51
D-arabinose
competitive inhibition, Ki: 50 mM
Sus scrofa
3.2.1.51
D-galactose
competitive inhibition, Ki: 1.7 mM
Sus scrofa
3.2.1.51
L-rhamnose
competitive inhibition, Ki: 140 mM
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.3
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
55000
-
4 * 55000, SDS-PAGE
Sus scrofa
3.2.1.51
192000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
Sus scrofa
-
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
Sus scrofa
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Sus scrofa
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Sus scrofa
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Sus scrofa
-
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Sus scrofa
?
3.2.1.51
thyroglobulin + H2O
Sus scrofa
hydrolysis only after pronase or neuraminidase treatment
alpha-L-fucose + ?
-
Sus scrofa
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Sus scrofa
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
-
Sus scrofa
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
thyroid gland
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
7.8
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208720
Sus scrofa
4-methylumbelliferone + alpha-L-fucose
-
208720
Sus scrofa
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208720
Sus scrofa
4-nitrophenol + alpha-L-fucose
-
208720
Sus scrofa
?
3.2.1.51
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
-
208720
Sus scrofa
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
208720
Sus scrofa
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208720
Sus scrofa
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208720
Sus scrofa
?
3.2.1.51
lacto-N-fucopentaose II + H2O
-
208720
Sus scrofa
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208720
Sus scrofa
?
3.2.1.51
submaxilary mucin trisaccharide + H2O
-
208720
Sus scrofa
alpha-L-fucose + disaccharide
-
208720
Sus scrofa
?
3.2.1.51
thyroglobulin + H2O
hydrolysis only after pronase or neuraminidase treatment
208720
Sus scrofa
alpha-L-fucose + ?
-
208720
Sus scrofa
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 55000, SDS-PAGE
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.1
-
-
Sus scrofa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
140
-
L-rhamnose
competitive inhibition
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate
complete inactivation at 22 mM
Sus scrofa
3.2.1.51
4-mercuribenzoate
complete inhibition at 0.25 mM
Sus scrofa
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 0.13 mM
Sus scrofa
3.2.1.51
D-arabinose
competitive inhibition, Ki: 50 mM
Sus scrofa
3.2.1.51
D-galactose
competitive inhibition, Ki: 1.7 mM
Sus scrofa
3.2.1.51
L-rhamnose
competitive inhibition, Ki: 140 mM
Sus scrofa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
140
-
L-rhamnose
competitive inhibition
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.3
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
55000
-
4 * 55000, SDS-PAGE
Sus scrofa
3.2.1.51
192000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
Sus scrofa
-
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
Sus scrofa
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Sus scrofa
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Sus scrofa
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Sus scrofa
-
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Sus scrofa
?
3.2.1.51
thyroglobulin + H2O
Sus scrofa
hydrolysis only after pronase or neuraminidase treatment
alpha-L-fucose + ?
-
Sus scrofa
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
-
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
thyroid gland
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.2.1.51
7.8
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208720
Sus scrofa
4-methylumbelliferone + alpha-L-fucose
-
208720
Sus scrofa
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208720
Sus scrofa
4-nitrophenol + alpha-L-fucose
-
208720
Sus scrofa
?
3.2.1.51
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
-
208720
Sus scrofa
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
208720
Sus scrofa
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208720
Sus scrofa
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208720
Sus scrofa
?
3.2.1.51
lacto-N-fucopentaose II + H2O
-
208720
Sus scrofa
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208720
Sus scrofa
?
3.2.1.51
submaxilary mucin trisaccharide + H2O
-
208720
Sus scrofa
alpha-L-fucose + disaccharide
-
208720
Sus scrofa
?
3.2.1.51
thyroglobulin + H2O
hydrolysis only after pronase or neuraminidase treatment
208720
Sus scrofa
alpha-L-fucose + ?
-
208720
Sus scrofa
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 55000, SDS-PAGE
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.1
-
-
Sus scrofa