BRENDA - Enzyme Database

The purification and characterization of alpha-L-fucosidase from hepatopancreas of Octopus vulgaris

D'Aniello, A.; Hakimi, J.; Cacace, G.M.; Ceccarini, C.; J. Biochem. 91, 1073-1080 (1982)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.358
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Octopus vulgaris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
70000
75000
gel filtration
Octopus vulgaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
glycopeptide + H2O
Octopus vulgaris
cellular source of substrate
alpha-L-fucose + ?
-
Octopus vulgaris
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Octopus vulgaris
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Octopus vulgaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
hepatopancreas
-
Octopus vulgaris
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
38.9
-
-
Octopus vulgaris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208718
Octopus vulgaris
4-nitrophenol + alpha-L-fucose
-
208718
Octopus vulgaris
?
3.2.1.51
glycopeptide + H2O
cellular source of substrate
208718
Octopus vulgaris
alpha-L-fucose + ?
-
208718
Octopus vulgaris
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.51
70
-
-
Octopus vulgaris
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
70
-
50% loss of activity after 5 min
Octopus vulgaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
3
-
one of two pH-optima
Octopus vulgaris
3.2.1.51
5.5
-
one of two pH-optima
Octopus vulgaris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.358
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Octopus vulgaris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
70000
75000
gel filtration
Octopus vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
glycopeptide + H2O
Octopus vulgaris
cellular source of substrate
alpha-L-fucose + ?
-
Octopus vulgaris
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Octopus vulgaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
hepatopancreas
-
Octopus vulgaris
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
38.9
-
-
Octopus vulgaris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208718
Octopus vulgaris
4-nitrophenol + alpha-L-fucose
-
208718
Octopus vulgaris
?
3.2.1.51
glycopeptide + H2O
cellular source of substrate
208718
Octopus vulgaris
alpha-L-fucose + ?
-
208718
Octopus vulgaris
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.51
70
-
-
Octopus vulgaris
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
70
-
50% loss of activity after 5 min
Octopus vulgaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
3
-
one of two pH-optima
Octopus vulgaris
3.2.1.51
5.5
-
one of two pH-optima
Octopus vulgaris