BRENDA - Enzyme Database

alpha-L-Fucosidases from almond emulsin

Kobata, A.; Methods Enzymol. 83, 625-631 (1982)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.1.51
60% loss of activity after lyophilization
Prunus dulcis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.095
-
lacto-N-fucopentaitol III
alpha-fucosidase I
Prunus dulcis
3.2.1.51
0.1
-
lacto-N-fucopentaitol II
alpha-fucosidase I
Prunus dulcis
3.2.1.51
0.67
-
2'-fucosyllactitol
alpha-fucosidase II
Prunus dulcis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
Prunus dulcis
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
alpha-L-fucose + lactitol
-
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
Prunus dulcis
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
Prunus dulcis
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
Prunus dulcis
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaitol III + H2O
Prunus dulcis
-
alpha-L-fucose + ?
-
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Prunus dulcis
alpha-fucosidase II
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Prunus dulcis
-
almond
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
partial, two forms
Prunus dulcis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
emulsin
two forms, alpha-fucosidase I and II
Prunus dulcis
-
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
frozen, more than 90% of its initial activity after 3 months
Prunus dulcis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
208717
Prunus dulcis
alpha-L-fucose + lactitol
-
208717
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc, alpha-(1-3)-GlcNAc or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I which has a Fuc-alpha-(1-2)-Gal-group
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
208717
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
208717
Prunus dulcis
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208717
Prunus dulcis
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208717
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaitol III + H2O
-
208717
Prunus dulcis
alpha-L-fucose + ?
-
208717
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaose II + H2O
alpha-fucosidase II
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208717
Prunus dulcis
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
6.5
alpha-fucosidase II
Prunus dulcis
3.2.1.51
5.5
-
alpha-fucosidase I
Prunus dulcis
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.1.51
60% loss of activity after lyophilization
Prunus dulcis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.095
-
lacto-N-fucopentaitol III
alpha-fucosidase I
Prunus dulcis
3.2.1.51
0.1
-
lacto-N-fucopentaitol II
alpha-fucosidase I
Prunus dulcis
3.2.1.51
0.67
-
2'-fucosyllactitol
alpha-fucosidase II
Prunus dulcis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
Prunus dulcis
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
alpha-L-fucose + lactitol
-
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
Prunus dulcis
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
Prunus dulcis
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
Prunus dulcis
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaitol III + H2O
Prunus dulcis
-
alpha-L-fucose + ?
-
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Prunus dulcis
alpha-fucosidase II
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
partial, two forms
Prunus dulcis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
emulsin
two forms, alpha-fucosidase I and II
Prunus dulcis
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
frozen, more than 90% of its initial activity after 3 months
Prunus dulcis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
208717
Prunus dulcis
alpha-L-fucose + lactitol
-
208717
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc, alpha-(1-3)-GlcNAc or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I which has a Fuc-alpha-(1-2)-Gal-group
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
208717
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
208717
Prunus dulcis
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208717
Prunus dulcis
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208717
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaitol III + H2O
-
208717
Prunus dulcis
alpha-L-fucose + ?
-
208717
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaose II + H2O
alpha-fucosidase II
208717
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208717
Prunus dulcis
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
6.5
alpha-fucosidase II
Prunus dulcis
3.2.1.51
5.5
-
alpha-fucosidase I
Prunus dulcis