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Literature summary extracted from
- alpha-L-Fucosidases from almond emulsin (1982), Methods Enzymol., 83, 625-631.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.51 | 60% loss of activity after lyophilization | Prunus dulcis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.51 | 0.095 | - |
lacto-N-fucopentaitol III | alpha-fucosidase I | Prunus dulcis |  |
| 3.2.1.51 | 0.1 | - |
lacto-N-fucopentaitol II | alpha-fucosidase I | Prunus dulcis | |
| 3.2.1.51 | 0.67 | - |
2'-fucosyllactitol | alpha-fucosidase II | Prunus dulcis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.51 | 2'-fucosyllactitol + H2O | Prunus dulcis | alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages | alpha-L-fucose + lactitol | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O | Prunus dulcis | alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group | alpha-L-fucose + Gal-beta-(1-4)-sorbitol | - |
? | |
| 3.2.1.51 | Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | Prunus dulcis | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| 3.2.1.51 | lacto-N-fucopentaitol III + H2O | Prunus dulcis | - |
alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | lacto-N-fucopentaose II + H2O | Prunus dulcis | alpha-fucosidase II | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.51 | Prunus dulcis | - |
almond | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.51 | partial, two forms | Prunus dulcis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.51 | emulsin | two forms, alpha-fucosidase I and II | Prunus dulcis | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.51 | frozen, more than 90% of its initial activity after 3 months | Prunus dulcis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.51 | 2'-fucosyllactitol + H2O | alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages | Prunus dulcis | alpha-L-fucose + lactitol | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O | alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc, alpha-(1-3)-GlcNAc or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I which has a Fuc-alpha-(1-2)-Gal-group | Prunus dulcis | alpha-L-fucose + Gal-beta-(1-4)-sorbitol | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O | alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group | Prunus dulcis | alpha-L-fucose + Gal-beta-(1-4)-sorbitol | - |
? | |
| 3.2.1.51 | Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | Prunus dulcis | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| 3.2.1.51 | Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | Prunus dulcis | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| 3.2.1.51 | lacto-N-fucopentaitol II + H2O | - |
Prunus dulcis | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | lacto-N-fucopentaitol III + H2O | - |
Prunus dulcis | alpha-L-fucose + ? | - |
? | |
| 3.2.1.51 | lacto-N-fucopentaose II + H2O | alpha-fucosidase II | Prunus dulcis | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.51 | 5 | 6.5 | alpha-fucosidase II | Prunus dulcis |
| 3.2.1.51 | 5.5 | - |
alpha-fucosidase I | Prunus dulcis |
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