BRENDA - Enzyme Database

Solubilization and characterization of pellet-associated human brain alpha-L-fucosidase activity

Hopfer, R.L.; Alhadeff, J.A.; Biochem. J. 229, 679-685 (1985)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.07
0.08
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
cytosol
-
Homo sapiens
5829
-
3.2.1.51
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
93800
-
membrane-bound form
Homo sapiens
3.2.1.51
109000
116000
gel filtration, soluble form
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
brain
-
Homo sapiens
-
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
-20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208716
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208716
Homo sapiens
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
30% loss of activity after 3 h
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
-
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.07
0.08
4-methylumbelliferyl-alpha-L-fucopyranoside
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
cytosol
-
Homo sapiens
5829
-
3.2.1.51
membrane
-
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
93800
-
membrane-bound form
Homo sapiens
3.2.1.51
109000
116000
gel filtration, soluble form
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
brain
-
Homo sapiens
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
-20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208716
Homo sapiens
4-methylumbelliferone + alpha-L-fucose
-
208716
Homo sapiens
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
30% loss of activity after 3 h
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
-
-
Homo sapiens