Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamamoto, K.; Tsuji, Y.; Kumagai, H.; Tochikura, T.
    Induction and purification of alpha-L-fucosidase from Fusarium oxysporum (1986), Agric. Biol. Chem., 50, 1689-1695.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 alpha-L-fucose 90% competitive inhibition at 5 mM, Ki: 0.12 mM Fusarium oxysporum
3.2.1.51 Hg2+ complete inhibition at 1 mM Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.48
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Fusarium oxysporum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.51 extracellular induction of enzyme synthesis by addition of L-fucose to the medium Fusarium oxysporum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 75000
-
 gel filtration Fusarium oxysporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 fucoidan + H2O Fusarium oxysporum
-
 alpha-L-fucose + ?
-
 ?
3.2.1.51 gastric mucin + H2O Fusarium oxysporum porcine alpha-L-fucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Fusarium oxysporum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein
-
 Fusarium oxysporum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 homogeneous, chromatography steps Fusarium oxysporum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 cell culture
-
 Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 110
-
-
 Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Fusarium oxysporum 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 fucoidan + H2O
-
 Fusarium oxysporum alpha-L-fucose + ?
-
 ?
3.2.1.51 gastric mucin + H2O porcine Fusarium oxysporum alpha-L-fucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 monomer 1 * 75000, SDS-PAGE Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 60
-
-
 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 45
-
 stable below Fusarium oxysporum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.51 4.5 6 optimal activity within Fusarium oxysporum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 5.5 10 stable within Fusarium oxysporum