BRENDA - Enzyme Database

Purification and characterization of alpha-L-fucosidase from Bacillus circulans grown on porcine gastric mucin

Tsuji, Y.; Yamamoto, K.; Tochikura, T.; Seno, T.; Ohkubo, Y.; Yamaguchi, H.; J. Biochem. 107, 324-330 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
Hg2+
potent inhibitor at 1 mM
Bacillus circulans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.63
-
2'-fucosyllactose
-
Bacillus circulans
3.2.1.51
0.88
-
gastric mucin
-
Bacillus circulans
3.2.1.51
1.25
-
3'-fucosyllactose
-
Bacillus circulans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
maximal enzyme synthesis by growth on porcine gastric mucin containing media
Bacillus circulans
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
135000
-
2 * 135000, SDS-PAGE
Bacillus circulans
3.2.1.51
285000
-
gel filtration
Bacillus circulans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Bacillus circulans
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Bacillus circulans
2'-fucosyllactose
alpha-L-fucose + lactose
-
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
Bacillus circulans
3-fucosyllactose
alpha-D-fucose + lactose
-
Bacillus circulans
?
3.2.1.51
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Bacillus circulans
lacto-N-fucopentaose III
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Bacillus circulans
?
3.2.1.51
gastric mucin + H2O
Bacillus circulans
porcine
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Bacillus circulans
-
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Bacillus circulans
?
3.2.1.51
lactoferrin + H2O
Bacillus circulans
low activity
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
milk + H2O
Bacillus circulans
human
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
orosomucoid + H2O
Bacillus circulans
low activity, higher activity if desialyzed
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
saliva + H2O
Bacillus circulans
human
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
submaxillary mucin + H2O
Bacillus circulans
A-minus type and desialyzed, porcine
alpha-L-fucose + ?
-
Bacillus circulans
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Bacillus circulans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, chromatography techniques
Bacillus circulans
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Bacillus circulans
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
20
-
-
Bacillus circulans
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
frozen, 1 year
Bacillus circulans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208708
Bacillus circulans
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208708
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208708
Bacillus circulans
alpha-L-fucose + lactose
-
208708
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208708
Bacillus circulans
alpha-L-fucose + lactose
-
208708
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208708
Bacillus circulans
alpha-D-fucose + lactose
-
208708
Bacillus circulans
?
3.2.1.51
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose III
208708
Bacillus circulans
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208708
Bacillus circulans
?
3.2.1.51
gastric mucin + H2O
porcine
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
lacto-N-fucopentaose II + H2O
-
208708
Bacillus circulans
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208708
Bacillus circulans
?
3.2.1.51
lactoferrin + H2O
low activity
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
milk + H2O
human
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
orosomucoid + H2O
low activity, higher activity if desialyzed
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
saliva + H2O
human
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
submaxillary mucin + H2O
A-minus type and desialyzed, porcine
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
dimer
2 * 135000, SDS-PAGE
Bacillus circulans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
-
Bacillus circulans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
45
-
stable up to
Bacillus circulans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
-
in citrate buffer
Bacillus circulans
3.2.1.51
6
6.5
in potassium phosphate buffer
Bacillus circulans
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4.5
9
stable within, kept at 4°C
Bacillus circulans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
Hg2+
potent inhibitor at 1 mM
Bacillus circulans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.63
-
2'-fucosyllactose
-
Bacillus circulans
3.2.1.51
0.88
-
gastric mucin
-
Bacillus circulans
3.2.1.51
1.25
-
3'-fucosyllactose
-
Bacillus circulans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
maximal enzyme synthesis by growth on porcine gastric mucin containing media
Bacillus circulans
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
135000
-
2 * 135000, SDS-PAGE
Bacillus circulans
3.2.1.51
285000
-
gel filtration
Bacillus circulans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Bacillus circulans
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Bacillus circulans
2'-fucosyllactose
alpha-L-fucose + lactose
-
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
Bacillus circulans
3-fucosyllactose
alpha-D-fucose + lactose
-
Bacillus circulans
?
3.2.1.51
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Bacillus circulans
lacto-N-fucopentaose III
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Bacillus circulans
?
3.2.1.51
gastric mucin + H2O
Bacillus circulans
porcine
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Bacillus circulans
-
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Bacillus circulans
?
3.2.1.51
lactoferrin + H2O
Bacillus circulans
low activity
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
milk + H2O
Bacillus circulans
human
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
orosomucoid + H2O
Bacillus circulans
low activity, higher activity if desialyzed
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
saliva + H2O
Bacillus circulans
human
alpha-L-fucose + ?
-
Bacillus circulans
?
3.2.1.51
submaxillary mucin + H2O
Bacillus circulans
A-minus type and desialyzed, porcine
alpha-L-fucose + ?
-
Bacillus circulans
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, chromatography techniques
Bacillus circulans
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Bacillus circulans
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
20
-
-
Bacillus circulans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
frozen, 1 year
Bacillus circulans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208708
Bacillus circulans
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208708
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208708
Bacillus circulans
alpha-L-fucose + lactose
-
208708
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208708
Bacillus circulans
alpha-L-fucose + lactose
-
208708
Bacillus circulans
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208708
Bacillus circulans
alpha-D-fucose + lactose
-
208708
Bacillus circulans
?
3.2.1.51
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose III
208708
Bacillus circulans
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208708
Bacillus circulans
?
3.2.1.51
gastric mucin + H2O
porcine
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
lacto-N-fucopentaose II + H2O
-
208708
Bacillus circulans
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208708
Bacillus circulans
?
3.2.1.51
lactoferrin + H2O
low activity
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
milk + H2O
human
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
orosomucoid + H2O
low activity, higher activity if desialyzed
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
saliva + H2O
human
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
3.2.1.51
submaxillary mucin + H2O
A-minus type and desialyzed, porcine
208708
Bacillus circulans
alpha-L-fucose + ?
-
208708
Bacillus circulans
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
dimer
2 * 135000, SDS-PAGE
Bacillus circulans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
-
Bacillus circulans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
45
-
stable up to
Bacillus circulans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5.5
-
in citrate buffer
Bacillus circulans
3.2.1.51
6
6.5
in potassium phosphate buffer
Bacillus circulans
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4.5
9
stable within, kept at 4°C
Bacillus circulans