BRENDA - Enzyme Database

Purification and properties of a secreted and developmentally regulated alpha-L-fucosidase from Dictyostelium discoideum

Schopohl, D.; Muller-Taubenberger, A.; Orthen, B.; Hess, H.; Reutter, W.; J. Biol. Chem. 267, 2400-2405 (1992)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
1.2
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Dictyostelium discoideum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
increasing activity up to aggregation stage, not detectable in the growth phase
Dictyostelium discoideum
-
-
3.2.1.51
lysosome
-
Dictyostelium discoideum
5764
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
62000
-
gel filtration
Dictyostelium discoideum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
Dictyostelium discoideum
low activity
alpha-L-fucose + lactitol
-
Dictyostelium discoideum
?
3.2.1.51
3-fucosyllactitol + H2O
Dictyostelium discoideum
very low activity
alpha-L-fucose + lactitol
-
Dictyostelium discoideum
?
3.2.1.51
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
Dictyostelium discoideum
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
alpha-L-fucopyranose + N-acetyl-glucosamine
-
Dictyostelium discoideum
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Dictyostelium discoideum
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
-
Dictyostelium discoideum
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Dictyostelium discoideum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Dictyostelium discoideum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
5
-
-
Dictyostelium discoideum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
low activity
208704
Dictyostelium discoideum
alpha-L-fucose + lactitol
-
208704
Dictyostelium discoideum
?
3.2.1.51
3-fucosyllactitol + H2O
very low activity
208704
Dictyostelium discoideum
alpha-L-fucose + lactitol
-
208704
Dictyostelium discoideum
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208704
Dictyostelium discoideum
4-nitrophenol + alpha-L-fucose
-
208704
Dictyostelium discoideum
?
3.2.1.51
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
208704
Dictyostelium discoideum
alpha-L-fucopyranose + N-acetyl-glucosamine
-
208704
Dictyostelium discoideum
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
monomer
1 * 62000, SDS-PAGE
Dictyostelium discoideum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
3.7
-
-
Dictyostelium discoideum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
1.2
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Dictyostelium discoideum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
increasing activity up to aggregation stage, not detectable in the growth phase
Dictyostelium discoideum
-
-
3.2.1.51
lysosome
-
Dictyostelium discoideum
5764
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
62000
-
gel filtration
Dictyostelium discoideum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
Dictyostelium discoideum
low activity
alpha-L-fucose + lactitol
-
Dictyostelium discoideum
?
3.2.1.51
3-fucosyllactitol + H2O
Dictyostelium discoideum
very low activity
alpha-L-fucose + lactitol
-
Dictyostelium discoideum
?
3.2.1.51
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
Dictyostelium discoideum
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
alpha-L-fucopyranose + N-acetyl-glucosamine
-
Dictyostelium discoideum
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
-
Dictyostelium discoideum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Dictyostelium discoideum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Dictyostelium discoideum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
5
-
-
Dictyostelium discoideum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
low activity
208704
Dictyostelium discoideum
alpha-L-fucose + lactitol
-
208704
Dictyostelium discoideum
?
3.2.1.51
3-fucosyllactitol + H2O
very low activity
208704
Dictyostelium discoideum
alpha-L-fucose + lactitol
-
208704
Dictyostelium discoideum
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208704
Dictyostelium discoideum
4-nitrophenol + alpha-L-fucose
-
208704
Dictyostelium discoideum
?
3.2.1.51
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
208704
Dictyostelium discoideum
alpha-L-fucopyranose + N-acetyl-glucosamine
-
208704
Dictyostelium discoideum
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
monomer
1 * 62000, SDS-PAGE
Dictyostelium discoideum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
3.7
-
-
Dictyostelium discoideum