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Literature summary extracted from

  • Schopohl, D.; Muller-Taubenberger, A.; Orthen, B.; Hess, H.; Reutter, W.
    Purification and properties of a secreted and developmentally regulated alpha-L-fucosidase from Dictyostelium discoideum (1992), J. Biol. Chem., 267, 2400-2405.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 1.2
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.51 extracellular increasing activity up to aggregation stage, not detectable in the growth phase Dictyostelium discoideum
-
-
3.2.1.51 lysosome
-
 Dictyostelium discoideum 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 62000
-
 gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 2'-fucosyllactitol + H2O Dictyostelium discoideum low activity alpha-L-fucose + lactitol
-
 ?
3.2.1.51 3-fucosyllactitol + H2O Dictyostelium discoideum very low activity alpha-L-fucose + lactitol
-
 ?
3.2.1.51 6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O Dictyostelium discoideum Fuc-alpha-1,6-GlcNAc-linkage most susceptible alpha-L-fucopyranose + N-acetyl-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Dictyostelium discoideum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein
-
 Dictyostelium discoideum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, affinity chromatography Dictyostelium discoideum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 cell culture
-
 Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 5
-
-
 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 2'-fucosyllactitol + H2O low activity Dictyostelium discoideum alpha-L-fucose + lactitol
-
 ?
3.2.1.51 3-fucosyllactitol + H2O very low activity Dictyostelium discoideum alpha-L-fucose + lactitol
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Dictyostelium discoideum 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O Fuc-alpha-1,6-GlcNAc-linkage most susceptible Dictyostelium discoideum alpha-L-fucopyranose + N-acetyl-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 monomer 1 * 62000, SDS-PAGE Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 3.7
-
-
 Dictyostelium discoideum