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Literature summary extracted from

  • Gramer, M.J.; Schaffer, D.V.; Sliwkowski, M.B.; Goochee, C.F.
    Purification and characterization of alpha-L-fucosidase from Chinese hamster ovary cell culture supernatant (1994), Glycobiology, 4, 611-616.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.51 increased half-life to 23 h at 37°C and stability to repeated freezing and thawing by addition of 0.5 mg/ml bovine serum albumin Cricetulus griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 alpha-L-fucose competitive inhibition, Ki: 0.065 mM Cricetulus griseus
3.2.1.51 deoxyfuconojirimycin competitive inhibition, Ki: 0.000024 mM Cricetulus griseus
3.2.1.51 fucosylamine competitive inhibition, Ki: 0.014 mM Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.031
-
 4-methylumbelliferyl-alpha-L-fucopyranoside at pH 7.0 Cricetulus griseus
3.2.1.51 0.072
-
 4-methylumbelliferyl-alpha-L-fucopyranoside at pH 5.0 Cricetulus griseus
3.2.1.51 1.02
-
 gp120 oligosaccharide at pH 5.0 Cricetulus griseus
3.2.1.51 1.75
-
 Fuc-alpha-(1-6)-GlcNAc at pH 5.0 Cricetulus griseus
3.2.1.51 1.79
-
 2'-fucosyllactose at pH 5.0 Cricetulus griseus
3.2.1.51 1.9
-
 3'-fucosyllactose at pH 5.0 Cricetulus griseus
3.2.1.51 10.6
-
 2'-fucosyllactose at pH 7.0 Cricetulus griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.51 extracellular normally a lysosomal glycoprotein, released by secretion and/or cell lysis Cricetulus griseus
-
-
3.2.1.51 lysosome
-
 Cricetulus griseus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 51000
-
 SDS-PAGE Cricetulus griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Cricetulus griseus 2'-fucosyllactose alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O Cricetulus griseus low activity alpha-D-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O Cricetulus griseus 3-fucosyllactose alpha-D-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-6)-GlcNAc + H2O Cricetulus griseus
-
 alpha-L-fucose + N-acetyl-D-glucosamine
-
 ?
3.2.1.51 gp120 oligosaccharides + H2O Cricetulus griseus released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates alpha-L-fucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Cricetulus griseus
-
 Chinese hamster ovary cells
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein
-
 Cricetulus griseus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, affinity chromatography Cricetulus griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 cell culture
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 64.8
-
-
 Cricetulus griseus

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 -20°C, 0.5 mg/ml bovine serum albumin, 6 months Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 3'-fucosyllactose + H2O
-
 Cricetulus griseus alpha-L-fucose + ?
-
 ?
3.2.1.51 4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
 Cricetulus griseus 4-methylumbelliferone + alpha-L-fucose
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O 2'-fucosyllactose Cricetulus griseus alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O low activity Cricetulus griseus alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O 3-fucosyllactose Cricetulus griseus alpha-L-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O low activity Cricetulus griseus alpha-D-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O 3-fucosyllactose Cricetulus griseus alpha-D-fucose + lactose
-
 ?
3.2.1.51 Fuc-alpha-(1-6)-GlcNAc + H2O
-
 Cricetulus griseus alpha-L-fucose + N-acetyl-D-glucosamine
-
 ?
3.2.1.51 gp120 oligosaccharide + H2O
-
 Cricetulus griseus alpha-L-fucose + ?
-
 ?
3.2.1.51 gp120 oligosaccharides + H2O released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates Cricetulus griseus alpha-L-fucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 monomer 1 * 51000, SDS-PAGE Cricetulus griseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 37
-
 half-life: 1 h Cricetulus griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5
-
 optimal hydrolysis rate for 2'-fucosyllactose and Fuc-alpha-(1-6)-GlcNAc Cricetulus griseus
3.2.1.51 7
-
 optimal hydrolysis rate for 4-methylumbelliferyl-alpha-L-fucopyranoside Cricetulus griseus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.51 0.000024
-
 deoxyfuconojirimycin competitive inhibition Cricetulus griseus
3.2.1.51 0.014
-
 fucosylamine competitive inhibition Cricetulus griseus