BRENDA - Enzyme Database

Purification and characterization of alpha-L-fucosidase from Chinese hamster ovary cell culture supernatant

Gramer, M.J.; Schaffer, D.V.; Sliwkowski, M.B.; Goochee, C.F.; Glycobiology 4, 611-616 (1994)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.2.1.51
increased half-life to 23 h at 37°C and stability to repeated freezing and thawing by addition of 0.5 mg/ml bovine serum albumin
Cricetulus griseus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 0.065 mM
Cricetulus griseus
3.2.1.51
deoxyfuconojirimycin
competitive inhibition, Ki: 0.000024 mM
Cricetulus griseus
3.2.1.51
fucosylamine
competitive inhibition, Ki: 0.014 mM
Cricetulus griseus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.031
-
4-methylumbelliferyl-alpha-L-fucopyranoside
at pH 7.0
Cricetulus griseus
3.2.1.51
0.072
-
4-methylumbelliferyl-alpha-L-fucopyranoside
at pH 5.0
Cricetulus griseus
3.2.1.51
1.02
-
gp120 oligosaccharide
at pH 5.0
Cricetulus griseus
3.2.1.51
1.75
-
Fuc-alpha-(1-6)-GlcNAc
at pH 5.0
Cricetulus griseus
3.2.1.51
1.79
-
2'-fucosyllactose
at pH 5.0
Cricetulus griseus
3.2.1.51
1.9
-
3'-fucosyllactose
at pH 5.0
Cricetulus griseus
3.2.1.51
10.6
-
2'-fucosyllactose
at pH 7.0
Cricetulus griseus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
normally a lysosomal glycoprotein, released by secretion and/or cell lysis
Cricetulus griseus
-
-
3.2.1.51
lysosome
-
Cricetulus griseus
5764
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
51000
-
SDS-PAGE
Cricetulus griseus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Cricetulus griseus
2'-fucosyllactose
alpha-L-fucose + lactose
-
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
Cricetulus griseus
low activity
alpha-D-fucose + lactose
-
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
Cricetulus griseus
3-fucosyllactose
alpha-D-fucose + lactose
-
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
Cricetulus griseus
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Cricetulus griseus
?
3.2.1.51
gp120 oligosaccharides + H2O
Cricetulus griseus
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
alpha-L-fucose + ?
-
Cricetulus griseus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Cricetulus griseus
-
Chinese hamster ovary cells
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
-
Cricetulus griseus
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Cricetulus griseus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Cricetulus griseus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
64.8
-
-
Cricetulus griseus
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
-20°C, 0.5 mg/ml bovine serum albumin, 6 months
Cricetulus griseus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208701
Cricetulus griseus
4-methylumbelliferone + alpha-L-fucose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208701
Cricetulus griseus
alpha-L-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
low activity
208701
Cricetulus griseus
alpha-L-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208701
Cricetulus griseus
alpha-L-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
low activity
208701
Cricetulus griseus
alpha-D-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208701
Cricetulus griseus
alpha-D-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
-
208701
Cricetulus griseus
alpha-L-fucose + N-acetyl-D-glucosamine
-
208701
Cricetulus griseus
?
3.2.1.51
gp120 oligosaccharides + H2O
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
208701
Cricetulus griseus
alpha-L-fucose + ?
-
208701
Cricetulus griseus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
monomer
1 * 51000, SDS-PAGE
Cricetulus griseus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
37
-
half-life: 1 h
Cricetulus griseus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
-
optimal hydrolysis rate for 2'-fucosyllactose and Fuc-alpha-(1-6)-GlcNAc
Cricetulus griseus
3.2.1.51
7
-
optimal hydrolysis rate for 4-methylumbelliferyl-alpha-L-fucopyranoside
Cricetulus griseus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
0.000024
-
deoxyfuconojirimycin
competitive inhibition
Cricetulus griseus
3.2.1.51
0.014
-
fucosylamine
competitive inhibition
Cricetulus griseus
General Stability (protein specific)
EC Number
General Stability
Organism
3.2.1.51
increased half-life to 23 h at 37°C and stability to repeated freezing and thawing by addition of 0.5 mg/ml bovine serum albumin
Cricetulus griseus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competitive inhibition, Ki: 0.065 mM
Cricetulus griseus
3.2.1.51
deoxyfuconojirimycin
competitive inhibition, Ki: 0.000024 mM
Cricetulus griseus
3.2.1.51
fucosylamine
competitive inhibition, Ki: 0.014 mM
Cricetulus griseus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
0.000024
-
deoxyfuconojirimycin
competitive inhibition
Cricetulus griseus
3.2.1.51
0.014
-
fucosylamine
competitive inhibition
Cricetulus griseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.031
-
4-methylumbelliferyl-alpha-L-fucopyranoside
at pH 7.0
Cricetulus griseus
3.2.1.51
0.072
-
4-methylumbelliferyl-alpha-L-fucopyranoside
at pH 5.0
Cricetulus griseus
3.2.1.51
1.02
-
gp120 oligosaccharide
at pH 5.0
Cricetulus griseus
3.2.1.51
1.75
-
Fuc-alpha-(1-6)-GlcNAc
at pH 5.0
Cricetulus griseus
3.2.1.51
1.79
-
2'-fucosyllactose
at pH 5.0
Cricetulus griseus
3.2.1.51
1.9
-
3'-fucosyllactose
at pH 5.0
Cricetulus griseus
3.2.1.51
10.6
-
2'-fucosyllactose
at pH 7.0
Cricetulus griseus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
normally a lysosomal glycoprotein, released by secretion and/or cell lysis
Cricetulus griseus
-
-
3.2.1.51
lysosome
-
Cricetulus griseus
5764
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
51000
-
SDS-PAGE
Cricetulus griseus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Cricetulus griseus
2'-fucosyllactose
alpha-L-fucose + lactose
-
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
Cricetulus griseus
low activity
alpha-D-fucose + lactose
-
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
Cricetulus griseus
3-fucosyllactose
alpha-D-fucose + lactose
-
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
Cricetulus griseus
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Cricetulus griseus
?
3.2.1.51
gp120 oligosaccharides + H2O
Cricetulus griseus
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
alpha-L-fucose + ?
-
Cricetulus griseus
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.51
glycoprotein
-
Cricetulus griseus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Cricetulus griseus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Cricetulus griseus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
64.8
-
-
Cricetulus griseus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
-20°C, 0.5 mg/ml bovine serum albumin, 6 months
Cricetulus griseus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
-
208701
Cricetulus griseus
4-methylumbelliferone + alpha-L-fucose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208701
Cricetulus griseus
alpha-L-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
low activity
208701
Cricetulus griseus
alpha-L-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208701
Cricetulus griseus
alpha-L-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
low activity
208701
Cricetulus griseus
alpha-D-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
3-fucosyllactose
208701
Cricetulus griseus
alpha-D-fucose + lactose
-
208701
Cricetulus griseus
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
-
208701
Cricetulus griseus
alpha-L-fucose + N-acetyl-D-glucosamine
-
208701
Cricetulus griseus
?
3.2.1.51
gp120 oligosaccharides + H2O
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
208701
Cricetulus griseus
alpha-L-fucose + ?
-
208701
Cricetulus griseus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
monomer
1 * 51000, SDS-PAGE
Cricetulus griseus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
37
-
half-life: 1 h
Cricetulus griseus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
-
optimal hydrolysis rate for 2'-fucosyllactose and Fuc-alpha-(1-6)-GlcNAc
Cricetulus griseus
3.2.1.51
7
-
optimal hydrolysis rate for 4-methylumbelliferyl-alpha-L-fucopyranoside
Cricetulus griseus