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Literature summary extracted from

  • Focarelli, R.; Cacace, M.G.; Seraglia, R.; Rosati, F.
    A nonglycosylated, 68-kDa alpha-L-fucosidase is bound to the mollusc bivalve Unio elongatulus sperm plasma membrane and differs from a glycosylated 56-kDa form present in the seminal fluid (1997), Biochem. Biophys. Res. Commun., 234, 54-58.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.71
-
 4-nitrophenyl-alpha-L-fucopyranoside sperm-bound enzyme Unio elongatulus
3.2.1.51 0.91
-
 4-nitrophenyl-alpha-L-fucopyranoside gonad fluid enzyme Unio elongatulus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.51 plasma membrane
-
 Unio elongatulus 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 55970
-
 gonad fluid enzyme, mass spectrometry Unio elongatulus
3.2.1.51 55971
-
 1 * 55971, gonad fluid enzyme, 1 * 67847, sperm-bound enzyme, mass spectrometry Unio elongatulus
3.2.1.51 67847
-
 1 * 55971, gonad fluid enzyme, 1 * 67847, sperm-bound enzyme, mass spectrometry Unio elongatulus
3.2.1.51 67850
-
 sperm-bound enzyme, mass spectrometry Unio elongatulus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Unio elongatulus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.51 glycoprotein N-linked oligosaccharides only for the gonad fluid enzyme, sperm-bound enzyme not N- or O-linked glycosylated Unio elongatulus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, two forms, affinity chromatography Unio elongatulus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 gonad fluid Unio elongatulus
-
3.2.1.51 semen one form tightly bound to the sperm plasma membrane, one form present in the gonad fluid Unio elongatulus
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 4°C, 10 mM sodium phosphate, pH 5.5, 1 mM EDTA, 1 mM PMSF, one month Unio elongatulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Unio elongatulus 4-nitrophenol + alpha-L-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 monomer 1 * 55971, gonad fluid enzyme, 1 * 67847, sperm-bound enzyme, mass spectrometry Unio elongatulus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 50 60
-
 Unio elongatulus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5
-
 gonad fluid enzyme sharper pH dependence curve than the sperm-bound enzyme Unio elongatulus